Western blot was performed using Anti-EBP50 Monoclonal Antibody (Product # PA5-17045) and a 50 kDa band corresponding to EBP50 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of MCF-7(Lane 1), T-47D ( Lane 2), SK-BR-3 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), Hep G2 (Lane 6), and PC-12 ( Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).