Western blot analysis was performed on whole cell extracts (30 µg lysate) of NK-92 (Lane 1) and NK-92 treated with IL-2 (100 ng/mL for 48 hours) (Lane 2). The blot was probed with Anti-Granzyme B Polyclonal Antibody (Product # PA5-17457, 1:2500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 32 kDa band corresponding to Granzyme B was observed in the cell line tested and was enhanced upon treatment.