Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed)
Western Blot: Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed) [NBP1-73653] - Western Blot of Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody [FITC] (Pre-adsorbed). Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. All samples were reduced. Load: 50 ng per lane. Block for 30 min at RT. Primary Antibody: Guinea Porcine anti-Rabbit IgG (H+L) Secondary antibody [FITC] (Pre-adsorbed) 1:500 for 60 min at RT. Secondary antibody: Anti-Guinea Porcine IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab). Rabbit F(c) migrates slightly higher.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Guinea Pig anti-Rabbit IgG (H+L) Secondary Antibody [FITC] (Pre-adsorbed)
Available conjugates: Available conjugates: FITC, DyLight 350, PE/Cy7, DyLight 594, PE, DyLight 405, Janelia Fluor 646Specificity: This antibody was pre-adsorbed against Human, Goat, and Mouse Serum Proteins. No reaction was observed against Human, Goat and Mouse Serum Proteins.Sizes Available: 1 mg