TRAF2 Antibody PA5-17500 from Thermo Fisher Scientific

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Western blot was performed using Anti-TRAF2 Polyclonal Antibody, (Product # PA5-17500) and a 55 kDa band corresponding to TRAF2 was observed in the cell lines and mouse thymus tested except in colon tissue which has lower expression. Whole cell extracts (40 µg lysate) of HeLa (Lane 1), THP-1 (Lane 2), Jurkat (Lane 3), HEK-293 (Lane 4), Raji (Lane 5), Mouse thymus (Lane 6), Rat thymus (Lane 7), Mouse colon (Lane 8) and Rat colon (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
ICC/IF

Immunofluorescence analysis of TRAF2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TRAF2 Polyclonal Antibody (Product # PA5-17500) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization in cytoplasm. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
TRAF2 Antibody

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TRAF2 Antibody

The TRAF2 Antibody from Thermo Fisher Scientific is a Rabbit Polyclonal antibody to TRAF2. This antibody recognizes Homo Sapiens, Human, Mouse, and Non-Human Primate antigen. The TRAF2 Antibody has been shown to work in the following applications: Immunocytochemistry, Immunoprecipitation, and Western Blot.

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TRAF2 Antibody

TRAF2 Antibody from Thermo Fisher Scientific

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TRAF2 Antibody from Thermo Fisher Scientific

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