CD3 epsilon Antibody (UCHT1)

Western Blot: CD3 epsilon Antibody (UCHT1) [NB100-2726] - Western blot shows cell lysates MOLT-4, Jurket, Raji, and THP-1. Membrane was probed with 2 ug/mL of Mouse Anti-CD3 epsilon Monoclonal Antibody (Catalog # NB100-2726) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD3 epsilon at approximately 21 kDa (as indicated) in the first 2 cell lysates but was absent from the later 2. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.

Western Blot: CD3 epsilon Antibody (UCHT1) [NB100-2726] - Western blot shows cell lysates MOLT-4, Jurket, Raji, and THP-1. Membrane was probed with 2 ug/mL of Mouse Anti-CD3 epsilon Monoclonal Antibody (Catalog # NB100-2726) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD3 epsilon at approximately 21 kDa (as indicated) in the first 2 cell lysates but was absent from the later 2. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.

Western Blot: CD3 epsilon Antibody (UCHT1) [NB100-2726] - Western blot shows cell lysates MOLT-4, Jurket, Raji, and THP-1. Membrane was probed with 2 ug/mL of Mouse Anti-CD3 epsilon Monoclonal Antibody (Catalog # NB100-2726) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD3 epsilon at approximately 21 kDa (as indicated) in the first 2 cell lysates but was absent from the later 2. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.