MYPT1 (D6C1) Rabbit mAb 8574S from Cell Signaling Technology

Product Specs
ItemMYPT1 (D6C1) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number8574S
Quantity100 µl
ApplicationsW, IP, IHC-P, IF-IC
ReactivityH, Mk, Dg
TargetMYPT1
HostRabbit
NCBI Gene AliasesGUBS, M130, MBS, MYPT1
ClonalityMonoclonal
NCBI Full Gene Nameprotein phosphatase 1 regulatory subunit 12A
Research AreaCytoskeletal Signaling
IsotypeIgG
CloneD6C1
Molecular Weight140

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Cell Signaling Technology

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Danvers, Massachusetts 01923

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Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) The FXR1 network acts as a signaling scaffold for actomyosin remodelingCellSeptember 5, 2024Chen X, Fansler MM, Janjoš U, Ule J, Mayr C.RRID:AB_922377" Mouse anti-ROCK1,Santa Cruz,"Cat# sc-17794, RRID:AB_628223" Rabbit anti-MYPT1,Cell Signaling,"Cat# 8574, RRID:AB_10998518" Rabbit anti-Phospho-MYPT1 (Thr853),Cell Signaling,"Cat# 4563, RRID:AB_1031185" Mouse

(2) Structure-dependent capacity of procyanidin dimers to inhibit inflammation-induced barrier dysfunction in a cell model of intestinal epitheliumRedox biologySeptember 1, 2024Zhu W, Xiong L, Oteiza PI.(#4370), ERK1/2 (#9102), p(Thr18/Ser19)-MLC (#3674), MLC (#8505), p(Thr696)-MYPT1 (#5163), MYPT1 (#8574), β-actin (#12620) and secondary antibody goat anti-rabbit IgG (#7074) were from Cell Signaling Technology

(3) Carvedilol Activates a Myofilament Signaling Circuitry to Restore Cardiac Contractility in Heart FailureJACC: Basic to Translational ScienceAugust 1, 2024Ying Wang, Meimi Zhao, Xianhui Liu, Bing Xu, Gopireddy R. Reddy, Aleksandra Jovanovic, Qingtong Wang, Chaoqun Zhu, Heli Xu, Elizabeth F. Bayne, et al.Cell Signaling Technology), anti–troponin I (1:1,000; 4002, Cell Signaling Technology), anti-MYPT1 (1:500; 8574, Cell Signaling Technology), anti-phospho-Akt2 serine 474 (1:1,000; 8599, Cell Signaling Technology),

(4) PPP1R12A is a recycling endosomal phosphatase that facilitates YAP activationScientific ReportsNovember 13, 2023Chiaki Inoue, Kojiro Mukai, Tatsuyuki Matsudaira, Jun Nakayama, Nozomu Kono, Junken Aoki, Hiroyuki Arai, Yasunori Uchida, Tomohiko Taguchi1:500), rabbit anti-YAP (#14074, dilution 1:100 for immunofluorescence), and rabbit anti-PPP1R12A (#8574, dilution 1:1000 for western blot, 1:200 for immunofluorescence) from Cell Signaling Technology; rabbit

(5) NUAK1 governs centrosome replication in pancreatic cancer via MYPT1/PP1β and GSK3β-dependent regulation of PLK4Molecular oncologyJuly 1, 2023Whyte D, Skalka G, Walsh P, Wilczynska A, Paul NR, Mitchell C, Nixon C, Clarke W, Bushell M, Morton JP, et al.used at the following dilutions – NUAK1 (CST 4458, 1 : 500); pMYPT1 (MRC S5087, 1 : 400), MYPT1 (CST 8574 1 : 1000), PLK4 (CST 71033, 1 : 500), pGSK S9 (CST 5558, 1 : 500), β‐Actin (Sigma‐Aldrich A5441, 1 : 5000

(6) Inhibition of growth and contraction in human prostate stromal cells by silencing of NUAK1 and -2, and by the presumed NUAK inhibitors HTH01-015 and WZ4003Frontiers in pharmacologyJanuary 1, 2023Liu Y, Wang R, Huang R, Rutz B, Ciotkowska A, Tamalunas A, Hu S, Trieb M, Waidelich R, Strittmatter F, et al.anti phospho-MYPT1 (Thr696) antibody (#5163, RRID:AB_10691830), rabbit monoclonal anti MYPT1 antibody (#8574, RRID:AB_10998518) (all from Cell Signaling Technology, Danvers, MA, United States), or mouse monoclonal

(7) MYPT1/PP1-Mediated EZH2 Dephosphorylation at S21 Promotes Epithelial-Mesenchymal Transition in Fibrosis through Control of Multiple Families of GenesAdvanced science (Weinheim, Baden-Wurttemberg, Germany)May 1, 2022Zhang L, Wang L, Hu XB, Hou M, Xiao Y, Xiang JW, Xie J, Chen ZG, Yang TH, Nie Q, et al.immunoprecipitation with EZH2 antibody (Cell Signaling Technology#5246) or MYPT1 antibody (Cell Signaling Technology #8574) as well as protein A/G agarose beads overnight at 4 °C. The immunoprecipitates were washed six times

(8) TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stressCell death & diseaseJanuary 7, 2021Guo S, Chen Y, Yang Y, Zhang X, Ma L, Xue X, Qiao Y, Wang J.anti-POMP (15141, 1:1000), anti-HIF1α (36169, 1:1000), anti-HIF1α-OH (3434, 1:1000), and anti-MYPT1 (8574, 1:1000), all purchased from Cell Signaling Technology (CST, Boston, MA, USA); anti-GFP (AG281, 1:1000)

(9) Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of TranscriptionMolecular cellMarch 19, 2020Cossa G, Roeschert I, Prinz F, Baluapuri A, Silveira Vidal R, Schülein-Völk C, Chang YC, Ade CP, Mastrobuoni G, Girard C, et al.,Cell Signaling Technology,Cat# 3724 RRID:AB_1549585 Lot: 7 Rabbit monoclonal anti-MYPT1,Cell Signaling Technology,Cat# 8574 RRID:AB_10998518 Lot: 1 Rabbit polyclonal anti-NUAK1 (used for immunoblotting),Cell Signaling Technology,Cat#

(10) Mechanisms of thrombin-Induced myometrial contractions: Potential targets of progesteronePloS oneJanuary 1, 2020Nishimura F, Mogami H, Moriuchi K, Chigusa Y, Mandai M, Kondoh E.Phospho-MYPT1 (Thr853, Cell Signaling, #4563, RRID: AB_1031185, 1:1000) MYPT1 (D6C1, Cell Signaling, #8574, RRID: AB_10998518, 1:1000) or anti-beta actin (Abcam, ab8227, 1:2000). All of the first antibodies

(11) (-)-Epicatechin and NADPH oxidase inhibitors prevent bile acid-induced Caco-2 monolayer permeabilization through ERK1/2 modulationRedox biologyJanuary 1, 2020Wang Z, Litterio MC, Müller M, Vauzour D, Oteiza PI.p65 (#8242), phospho-MLC 2 (Ser19) (#3671), MLC 2 (#8505), phospho-MYPT1 (Thr696) (#5163); MYPT1 (#8574), phospho-EGFR (Tyr1068) (#3777), EGFR (#4267), and β-actin (#12620) were from Cell Signaling Technology

(12) TIMAP inhibits endothelial myosin light chain phosphatase by competing with MYPT1 for the catalytic protein phosphatase 1 subunit PP1cβThe Journal of biological chemistrySeptember 6, 2019Wang X, Obeidat M, Li L, Pasarj P, Aburahess S, Holmes CFB, Ballermann BJ.monoclonal rabbit anti-MLC2 antibody (catalog no. 8505), and monoclonal rabbit anti-MYPT1 (catalog no. 8574) were from Cell Signaling Technology (New England Biolabs, Inc.). Monoclonal mouse anti-β actin antibody

(13) Role of the Rho/ROCK signaling pathway in the protective effects of fasudil against acute lung injury in septic ratsMolecular medicine reportsNovember 1, 2018Wang Y, Wang X, Liu W, Zhang L.1:500; both Abcam) or rabbit monoclonal anti-myosin phosphatase targeting subunit 1 (MYPT-1; cat. no. 8574; 1:1,000), monoclonal anti-phosphorylated (p)-MYPT-1 (cat. no. 4563; 1:1,000; both Cell Signaling Technology,

(14) DGC-specific RHOA mutations maintained cancer cell survival and promoted cell migration via ROCK inactivationOncotargetMay 1, 2018Takashi Nishizawa, Kiyotaka Nakano, Aya Harada, Miwako Kakiuchi, Shin-Ichi Funahashi, Masami Suzuki, Shumpei Ishikawa, Hiroyuki Aburatani(Cell Signaling, #2117, #2098, #3430, #3313, #5175, #3674, #8505, #3841, #3842, #3845, #5163, #4563, #8574), and ACTB (Sigma-Aldrich, A1978). ACTB was used as an internal control. Structural analysis The homology

(15) Calcium signalling links MYC to NUAK1OncogeneFebruary 1, 2018Tiziana Monteverde, Jacqueline Tait-Mulder, Ann Hedley, John R. Knight, Owen J. Sansom, Daniel J. Murphyphospho-Ser792(#2083), total Raptor (#3661), AMPK phospho-T172(#2535), total AMPK (#2532), total MYPT1 (#8574), PKCα (#2056), NUAK1 (#4458), phospho-(Ser) PKC substrate (#2261), MARCKS phospho-Ser159/163 (#11992)

(16) Semaphorin 7A Promotes VEGFA/VEGFR2-Mediated Angiogenesis and Intraplaque Neovascularization in ApoE^-/- MiceFrontiers in physiologyJanuary 1, 2018Hu S, Liu Y, You T, Zhu L.rabbit anti-human ROCK1 (3033, Cell Signaling Technology, United States), and rabbit anti-human MYPT1 (8574, Cell Signaling Technology, United States). After incubation at 4°C overnight and TBST washing, membranes

(17) Platelets reduce anoikis and promote metastasis by activating YAP1 signalingNature communicationsAugust 21, 2017Haemmerle M, Taylor ML, Gutschner T, Pradeep S, Cho MS, Sheng J, Lyons YM, Nagaraja AS, Dood RL, Wen Y, et al.cat. no. 3952, Cell Signaling), MOB1 (1:500, cat.no.13730, Cell Signaling), MYPT1 (1 : 1000, cat. no. 8574, Cell Signaling), Phospho-MYPT1 (1 : 1000, T853, cat. no. 4563, Cell Signaling), Phospho-MYPT1 (1 : 1000

(18) RhoD Inhibits RhoC-ROCK-Dependent Cell Contraction via PAK6Developmental cellMay 8, 2017Durkin CH, Leite F, Cordeiro JV, Handa Y, Arakawa Y, Valderrama F, Way M.antibody,Sigma-Aldrich,Cat#Z0134; RRID: AB_261899 Rabbit monoclonal MYPT1 (D6C1),Cell Signaling Technology,Cat#8574S; RRID: AB_10998518 Rabbit Phospho-MYPT1 (Thr696) Antibody,Cell Signaling Technology,Cat#5163S; RRID:

(19) Inhibition of ROCK1 kinase modulates both tumor cells and stromal fibroblasts in pancreatic cancerPloS oneJanuary 1, 2017Whatcott CJ, Ng S, Barrett MT, Hostetter G, Von Hoff DD, Han H.Materials and Methods section. The primary antibodies used include anti-MYPT1 from Cell Signaling (Cat #Cat 8574) at 1:750 dilution; antipMYPT1 (Thr696) from Cell Signaling (Cat #5163) at 1:750 dilution; and anti-β-actin

(20) Effects of phased joint intervention on Rho/ROCK expression levels in patients with portal hypertensionExperimental and Therapeutic MedicineSeptember 1, 2016Min Shi, Jue Wei, Wen-Ying Meng, Na Wang, Ting Wang, Yu-Gang Wang(67B9) mAb (cat. no. 2117), rabbit anti‑myosin phosphatase target subunit 1 (MYPT1) (D6C1) mAb (cat. no. 8574) and rabbit anti‑phosphorylated (p)‑MYPT1 (Thr853) polyclonal antibody (cat. no. 4563) were all purchased

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MYPT1 (D6C1) Rabbit mAb

MYPT1 (D6C1) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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MYPT1 (D6C1) Rabbit mAb from Cell Signaling Technology

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