Fig 1: The effect of blocking the key molecules of B cells on the production of anti-CD4 mAb in B cells of patients with INR. The B cells were identified and isolated from healthy and INR patients, and knockdown of BAFFR by lentivirus in these cells. A The knockdown efficiency of BAFFR was detected by Western blot. B Detection of BAFFR content by Flow cytometry. C Detection B cell viability by CCK-8. D Detection of anti-CD4 mAb content by ELISA. Data are represented as mean ± SD of 8 separate experiments. ***P < 0.001
Fig 2: Changes of B cell activity in clinical HIV patients. The patients were divided into four groups: health individuals, HIV-infected patients, ART patients, and INR. A Flow cytometric detection and isolation of B cells (CD19, CD20). B Flow cytometric detection of BAFFR, BCR, TLR and co-stimulatory molecules. C Western blot detected the protein expression of BAFFR, BCR, TLR and co-stimulatory molecules. D The content of anti-CD4 mAb was measured by ELISA. Data are represented as mean ± SD of 8 separate experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. the control or HIV-ART cohort
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