Fig 1: Piezo2 knockdown could reduce the angiogenic capacity of HUVECs. A: Matrigel tube formation assay to detect tube length; B: Matrigel tube formation assay to detect tube formation; C: CCK-8 assay to detect cell proliferation; D: Flow cytometry to detect cell cycle. Compared with the sh-NC group, * P < 0.05, ** P < 0.01, *** P < 0.001. Error bars represent standard deviation
Fig 2: Piezo2 was closely related to the Notch signaling pathway. A: RT-qPCR to detect Piezo2 mRNA levels; B: WB to detect Piezo2 protein levels; C: RT-qPCR to detect the mRNA levels of Notch pathway-related molecules; D: Immunofluorescence to detect the expression of Notch pathway-related molecules. Compared with the sh-NC group, * P < 0.05, ** P < 0.01, *** P < 0.001. Error bars represent standard deviation
Supplier Page from Abcam for Anti-PIEZO2 antibody [EPR27152-48]