CD44 Antibody AF6127 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCD44 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF6127
TargetCD44
IsotypeIgG
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Blockade of Receptor-ligand Interaction,Neutralization,Immunocytochemistry,CyTOF-ready
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
HostSheep
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenChinese hamster ovary cell line CHO-derived recombinant mouse CD44, Gln25-Thr224, Accession # NP_033981
ConcentrationLYOPH
PurityAntigen Affinity-purified
NCBI Full Gene NameCD44 molecule (Indian blood group), CD44 antigen
NCBI Gene AliasesMIC4, MDU2, HUTCH-1, Ly-24, IN, Pgp-1, CSPG8, H-CAM, MC56, Hermes-1, HUTCH-I, MDU3, LHR, ECM-III, ECMR-III, HCELL, Pgp1, HERMES, CDW44
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
ReactivityHu, Ms, Rt, Hr, Pg

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse and rat CD44 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human CD44 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study CD44 in mouse models of insulin resistance?

Yes, the CD44 antibody has been utilized in studies investigating its role in insulin resistance in mouse models, specifically in high-fat-fed C57BL/6 mice. Research has shown that CD44 is positively correlated with insulin resistance, and the antibody was employed to detect CD44 protein expression in these studies ().

Is this antibody effective for immunohistochemistry applications in cancer research?

The CD44 antibody has been successfully used in immunohistochemistry to assess CD44 expression in breast cancer tissues. It has been shown to correlate with the invasive ability of breast cancer cells, indicating its relevance in cancer research ().

What species can this antibody detect, and is it cross-reactive with human CD44?

This antibody is reactive with mouse, rat, horse, and porcine CD44, and it has approximately 35% cross-reactivity with recombinant human CD44 in direct ELISAs and Western blots, making it suitable for comparative studies across these species.

Has this antibody been cited in studies related to neurological conditions?

Yes, the CD44 antibody has been cited in research examining its role in epilepsy. A study demonstrated that CD44 deficiency in astrocytes reduced the severity of kainate-induced epilepsy, highlighting the antibody's application in neurological research ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Eplerenone improves kidney function and cardiac performance in obese male miceDiabetic MedicineOctober 3, 2025Ayman K. Banah, Bingxue Qi, Ryan Koh, Alexander Lawlor, Tianzhen Wang, Vishal Musale, Joanne Mains, Colin E. Murdoch, Li KangCell Signalling), AKT (9272, 1:1000; Cell Signalling), RHAMM/CD168(E7S4Y) (87129, 1:500; Cell Signalling), CD44 (AF6127; 1 μg/mL; R&D Systems), GAPDH (5174, 1:1000; Cell Signalling), beta‐tubulin (ab6046, 1:1000; Abcam)

(2) Limiting extracellular matrix expansion in diet-induced obese mice reduces cardiac insulin resistance and prevents myocardial remodellingMolecular metabolismAugust 1, 2024Musale V, Murdoch CE, Banah AK, Hasib A, Hennayake CK, Dong B, Lang CC, Wasserman DH, Kang L.per sample was loaded onto 4–12% SDS-PAGE gels for protein detection, using antibodies against CD44 (AF6127, 1:1,000; R&D Systems), RHAMM (87129, 1:1000, Cell Signalling), TGF-β (ab179695, 1:1000, Abcam), Phospho-Smad2

(3) Extracellular Matrix Abnormalities Contribute to Cardiac Insulin Resistance and Associated Dysfunction in Diet-induced Obese MicebioRxivNovember 21, 2023Vishal Musale, Colin E. Murdoch, Ayman K. Banah, Annie Hasib, Chandani K. Hennayake, Bo Dong, Chim C. Lang, David H. Wasserman, Li Kangper sample was loaded onto 4–12% SDS-PAGE gels for protein detection, using antibodies against CD44 (AF6127, 1:1,000; R&D Systems), RHAMM (87129, 1:1000, Cell Signalling), TGF-β (ab179695, 1:1000, Abcam), Phospho-Smad2

(4) Astrocytic CD44 Deficiency Reduces the Severity of Kainate-Induced EpilepsyCellsMay 26, 2023Kruk PK, Nader K, Skupien-Jaroszek A, Wójtowicz T, Buszka A, Olech-Kochańczyk G, Wilczynski GM, Worch R, Kalita K, Włodarczyk J, et al.performed according to a standard procedure, with anti-CD44 antibody (R&D Systems, Minneapolis, MN, USA, AF6127; 1:2500) and anti-GAPDH antibody (Abcam, Cambridge, UK, ab9485; 1:5000), with the latter being used

(5) Negative regulation of CD44st by miR-138-5p affects the invasive ability of breast cancer cells and patient prognosis after breast cancer surgeryBMC cancerMarch 24, 2023Jian FX, Bao PX, Li WF, Cui YH, Hong HG.corresponding negative control (NC-miR, 5ʹ-GCGGUCGUGCAGUGCGUGAUAUA-3ʹ) was synthesized by RiboBio Inc. CD44 (AF6127), CD44s (BBA10), and CD44v6 (BBA13) antibodies were purchased from R&D systems (USA). Transwell assays

(6) Hyaluronan supports the limbal stem cell phenotype during ex vivo cultureStem cell research & therapyJuly 30, 2022Puri S, Moreno IY, Sun M, Verma S, Lin X, Gesteira TF, Coulson-Thomas VJ.mechanism by which HA supports LESCs, anti-CD44 neutralizing antibody (R&D systems, Minneapolis, USA#AF6127) was added to the culture media in order to block the binding of HA to CD44. Importantly, CD44 contains

(7) SYK-3BP2 Pathway Activity in Parenchymal and Myeloid Cells Is a Key Pathogenic Factor in Metabolic SteatohepatitisCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Luci C, Vieira E, Bourinet M, Rousseau D, Bonnafous S, Patouraux S, Lefevre L, Larbret F, Prod'homme V, Iannelli A, et al.described.53 The proteins were probed with Anti-SYK (#2712; Cell Signaling Technology), Anti-CD44 (AF6127; Bio-Techne), Anti-phospho SYK (Tyr525/526) (#2710; Cell Signaling Technology), Anti-p44/42 MAPK (#4695;

(8) SYK-3BP2 Pathway Activity in Parenchymal and Myeloid Cells Is a Key Pathogenic Factor in Metabolic SteatohepatitisCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Luci C, Vieira E, Bourinet M, Rousseau D, Bonnafous S, Patouraux S, Lefevre L, Larbret F, Prod'homme V, Iannelli A, et al.described.53 The proteins were probed with Anti-SYK (#2712; Cell Signaling Technology), Anti-CD44 (AF6127; Bio-Techne), Anti-phospho SYK (Tyr525/526) (#2710; Cell Signaling Technology), Anti-p44/42 MAPK (#4695;

(9) Effect of exosomes from adipose-derived stem cells on the apoptosis of Schwann cells in peripheral nerve injuryCNS neuroscience & therapeuticsFebruary 1, 2020Liu CY, Yin G, Sun YD, Lin YF, Xie Z, English AW, Li QF, Lin HD.

(10) CD44 contributes to hyaluronan-mediated insulin resistance in skeletal muscle of high-fat-fed C57BL/6 miceAmerican journal of physiology. Endocrinology and metabolismDecember 1, 2019Hasib A, Hennayake CK, Bracy DP, Bugler-Lamb AR, Lantier L, Khan F, Ashford MLJ, McCrimmon RJ, Wasserman DH, Kang L.following antibodies were used to detect respective proteins using Western blotting: CD44 (cat. no. AF6127, 1:1,000; R&D Systems, UK) (21); p-Akt(Ser473) (9), p-Akt(Thr308) (9), total Akt (9), p-GSK-3␤(Ser9)

(11) Deconstructed Microfluidic Bone Marrow On-A-Chip to Study Normal and Malignant Hemopoietic Cell-Niche InteractionsSmall (Weinheim an der Bergstrasse, Germany)October 1, 2019Aleman J, George SK, Herberg S, Devarasetty M, Porada CD, Skardal A, Almeida-Porada G.anti-human EphB4 (37–1800; Thermo Fisher Scientific, Waltham, MA), sheep antimouse/rat/porcine/equine CD44 (AF6127; R&D Systems, Minneapolis, MN), and mouse anti-human Stro-1 (MAB1038; R&D Systems, Minneapolis, MN).

(12) Serglycin is involved in inflammatory response in articular mouse chondrocytesBiochemical and biophysical research communicationsMay 15, 2018D'Ascola A, Scuruchi M, Avenoso A, Bruschetta G, Campo S, Mandraffino G, Campo GM.

(13) Single-cell topological RNA-seq analysis reveals insights into cellular differentiation and developmentNature biotechnologyJune 1, 2017Rizvi AH, Camara PG, Kandror EK, Roberts TJ, Schieren I, Maniatis T, Rabadan R.(BD Biosciences 611202, 1:100), goat-αPECAM1 (Santa Cruz sc-1506, 1:100), sheep-αCD44 (R&D Systems AF6127, 1:250), goat-αFoxc1 (Santa Cruz sc-21396, 1:50), and goat-αSstr2 (Santa Cruz sc-11606, 1:50). Alexa

(14) Deficient TSC1/TSC2-complex suppression of SOX9-osteopontin-AKT signalling cascade constrains tumour growth in tuberous sclerosis complexHuman molecular geneticsJanuary 15, 2017Jin F, Jiang K, Ji S, Wang L, Ni Z, Huang F, Li C, Chen R, Zhang H, Hu Z, et al.

(15) Immunogenomics reveal molecular circuits of diclofenac induced liver injury in miceOncotargetMarch 22, 2016Lee EH, Oh JH, Selvaraj S, Park SM, Choi MS, Spanel R, Yoon S, Borlak J.,Santa Cruz Biotechnology,sc-482,I1113 TC-PTP (S-17),Santa Cruz Biotechnology,sc-102129, CD44,R & D SYSTEMS,AF6127,

(16) Uncovering the dual role of RHAMM as an HA receptor and a regulator of CD44 expression in RHAMM-expressing mesenchymal progenitor cellsFrontiers in cell and developmental biologyJanuary 1, 2015Veiseh M, Leith SJ, Tolg C, Elhayek SS, Bahrami SB, Collis L, Hamilton S, McCarthy JB, Bissell MJ, Turley E.previously described (Tolg et al., 2006), with the exception of using primary anti-CD44 antibodies (AF6127, R&D systems) at 1 µg/mL overnight. Secondary antibodies were incubated for 1 h at room temperature

(17) Recombinant human hyaluronidase PH20 does not stimulate an acute inflammatory response and inhibits lipopolysaccharide-induced neutrophil recruitment in the air pouch model of inflammationJournal of immunology (Baltimore, Md. : 1950)June 1, 2014Huang Z, Zhao C, Chen Y, Cowell JA, Wei G, Kultti A, Huang L, Thompson CB, Rosengren S, Frost GI, et al.

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CD44 Antibody

CD44 Antibody

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CD44 Antibody

CD44 Antibody

CD44 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

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CD44 Antibody from R&D Systems, a Bio-Techne Brand

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