Fig 1: Adipose tissue inflammation and gene expression in PDE3B-/-mice.WAT was collected from SvJ129 WT and PDE3B-/-mice. (a) mRNA expressions of inflammatory cytokines (IL1ß, TNFa, IFN?) were analyzed using qRT-PCR. (N = 5 WT and 5 PDE3B-KO mice) and presented as fold-up/down-regulation of expression of mRNA levels. (b) Downregulation of major inflammatory chemokines. Experiment conducted using Mouse Inflammasome Profiler PCR Array (Qiagen) and depicted here as fold-up/down-regulation of mRNA levels. Results are presented as relative mRNA fold change (fold change+/-SE) compared to WT mice (N = 4 WT and 4 PDE3B-/-mice). (c) Protein expressions of inflammatory cytokines (IL1ß, TNFa, IFN?), CD80, CD11c and NOS2 were analyzed using Western blots. HSP90 protein levels were used as loading control. Results are presented as relative protein fold change compared to WT mice. All bar graphs represent fold change+/-SE (n = 2 independent experiments, each experiment with samples from 6 WT and 6 PDE3B-/- mice adipose tissue lysates).