Western blot was performed using Anti-PDHA1/PDHA2 Polyclonal Antibody (Product # PA5-17190) and a band at ~43 kDa corresponding to PDH was observed in all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK 293 (Lane 2), MDA-MB-231 (Lane 3), HL-60 (Lane 4), SK-OV-3 (Lane 5), PC-3 (Lane 6), PANC-1 (Lane 7), Mouse Liver (Lane 8), Rat Liver (Lane 9), Mouse Brown Adipose (Lane 10), Mouse Brain (Lane 11) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).