beta Tubulin Antibody PA5-16863 from Thermo Fisher Scientific

Product Specs
Itembeta Tubulin Antibody
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberPA5-16863
HostRabbit
Quantity500 µL
IsotypeIgG
NCBI Gene Aliases2810484G07Rik, CDCBM, 2410129E14Rik, RGD1309427, MACTHC1, Tubb, M(beta)4, 2310057H16Rik, FPVEPD, Tubb2b, CFEOM3A, M(beta)2, M(beta)3, CSCSC1, M(beta)1, M(beta)6, 3200002H15Rik, M(beta)5, TUBB-5, OOMD2, PMGYSA, brdp, TUBB4, OOMD, beta-5, M40, TUBB5, TUBB2, CDCBM5, CFEOM3, bA506K6.1, bA631M21.2, HsT1601, TUBB1, CDCBM1, RGD1305887, CDCBM7, CDCBM6, OZEMA2, Tubb4, Tubb2, TUBB, DYT4, B130022C14Rik, OK/SW-cl.56, FEOM3, beta-4
FormatPBS with 1% BSA and 0.1% sodium azide; pH 7.6
ReactivityHuman, Mouse, Rat
Conjugate/TagUnconjugated
Targetbeta Tubulin
ApplicationsImmunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
ClonalityPolyclonal
NCBI Full Gene Nametubulin, beta 6 class V, tubulin beta 6 class V, tubulin, beta 4A class IVA, tubulin, beta 4A class IVa, tubulin, beta 5 class I, tubulin beta 3 class III, tubulin, beta 3 class III, tubulin, beta 2A class IIA, tubulin beta 4A class IVa, tubulin beta 1 class VI, tubulin beta 2B class IIb, tubulin beta class I, tubulin beta 8 class VIII, tubulin beta 2A class IIa, tubulin, beta 2B class IIb, tubulin, beta 1 class VI, tubulin, beta 2A class IIa, tubulin, beta 2B class IIB
ImmunogenA synthetic peptide derived from aa 416-430 of human tubulin beta
Storage Temperature4° C
PurityAntigen affinity chromatography
Antibody TypePrimary
Concentration0.1 mg/mL
Storage-20°C, Avoid Freeze/Thaw Cycles

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Development of a Breast-on-a-Chip Microfluidic Model to Assess the Effect of Palbociclib in MCF-7 and T47D Cancer CellsCellsMarch 2, 2026Ingrid Larissa Melo Souza, Ana Cláudia Martins Braga Gomes Torres, Rodrigo Lucas, Isabella Gizzi Jiacomini, Sthefanie Ribas Klein, Maíra Barbosa E Reis, Andréia Akemi Suzukawa, Dalila Lucíola Zanette, Mateus Nóbrega Aoki, Alessandra Melo de Aguiar, et al.at room temperature. MCF-7 and T47D cytoskeletons were incubated with anti-β-tubulin antibody (cat. PA5-16863, Invitrogen) overnight under shaking, followed by anti-rabbit Alexa FluorTM 488 donkey anti-rabbit IgG

(2) The pursuit of novel head and neck cancer biomarkers - tissue and blood expression of chloride intracellular channels familyPLoS OneOctober 24, 2025Bartosz Paweł Wojtera, Kamila Ostrowska, Julia Ostapowicz, Mateusz Szewczyk, Julia Kozikowska, Wiktoria Maria Suchorska, Wojciech Golusiński(Thermo Fisher Scientific Cat# 31460, RRID: AB_228341). Rp anti-beta-tubulin Ab (Thermo Fisher Scientific Cat# PA5–16863, RRID: AB_10986058, Waltham, MA, USA) was used as the reference protein. Bands were visualized using

(3) EZH2 Inhibition in Mesothelioma Cells Increases the Release of Extracellular Vesicles That Skew Neutrophils Toward a Protumor PhenotypeInternational Journal of Molecular SciencesOctober 23, 2025Giulia Pinton, Elia Bari, Silvia Fallarini, Valentina Gigliotti, Veronica De Giorgis, Fausto Chiazza, Maria Luisa Torre, Marcello Manfredi, Laura MoroUSA). The polyclonal antibodies specific for CD63 (PA5-92370), RAB27b (PA5-54096) and beta-Tubulin (PA5-16863) were from Thermo Fisher Scientific. Anti-PD-L1 (clone MIH2), anti-CXCR4 (clone QA18A64), anti-CD62L

(4) Proteomic Investigation of Neurotrophic trans-Banglene Reveals Potential Link to Iron HomeostasisMolecular neurobiologyJuly 1, 2025Gunawardana PBW, Gohil K, Moon KM, Foster LJ, Williams FJ.beta Actin Polyclonal Antibody (Invitrogen™ PA116889), beta Tubulin Polyclonal Antibody, (Invitrogen™ PA516863), Phospho-AKT1 (Thr308) Recombinant Polyclonal Antibody (B18HCLC) (Invitrogen™ 710,122), Anti-MEF2C

(5) NeuroD2 Modulates Hippocampal Neuronal Organization and Axon Guidance During AgingIn VivoJuly 1, 2025MERVE BEKER, BUSENUR BOLAT, BAHAR SARIKAMIS JOHNSON, NILUFER ERCIN, RABIA KALKAN CAKMAK, SARE B. KAYA, NAIL BESLI, ULKAN CELIK, MUSTAFA C. BEKERiBright device (Thermo Fisher Scientific). The membranes were stripped and re-probed against β-tubulin (PA5-16863, Thermo Fisher Scientific) and GAPDH (2118, Cell Signaling Technologies) for normalization. The ImageJ

(6) Characterization of senescence and nuclear reorganization in aging gingival cellsNPJ AgingFebruary 21, 2025Christian Fernández, Diego Ormeno, Verónica Villalobos, Mauricio Garrido, Javiera Canelo, Oscar Cerda, Felipe Maldonado, Mónica CaceresNectin-2/CD112 (RyD Systems catalog #MAB2229) [1/1000], and Anti-beta Tubulin (Thermo Fisher Scientific catalog #PA5-16863) [1/5000]. After 3 washes with 0.1% v/vTweeen-20/TBS, the membranes were incubated with the appropriate

(7) Molecular basis promoting centriole triplet microtubule assemblyNature communicationsMarch 22, 2024Takeda Y, Chinen T, Honda S, Takatori S, Okuda S, Yamamoto S, Fukuyama M, Takeuchi K, Tomita T, Hata S, et al.

(8) Bioresponsive and immunotherapeutic nanomaterials to remodel tumor microenvironment for enhanced immune checkpoint blockadeBioactive materialsFebruary 1, 2024Fang T, Cao X, Wang L, Chen M, Deng Y, Chen G.corresponding primary antibodies (HIF-1α: NOVUS, Cat no. NB100-654; β-tubulin: Invitrogen, Cat no. PA5-16863) and the secondary antibody (Invitrogen, horseradish peroxidase (HRP)-labeled, Cat no. 65–1620) and

(9) Inducible tricolor reporter mouse for parallel imaging of lysosomes, mitochondria, and microtubulesThe Journal of cell biologyJanuary 1, 2024Hutchison V, Lynch A, Gutierrez-Gamez AM, Chen J.,Thermo Fisher Scientific,Rat,MA1-80017,"1:1,000",IF β actin,Thermo Fisher Scientific,Rabbit,MA5-32540,"1:1,000",WB β tubulin,Invitrogen,Rabbit,PA5-16863,"1:1,000",IF β tubulin,Invitrogen,Rabbit,PA5-16863,1:200,WB Car4,R&D Systems,Goat,AF2414,"1:1,000",IF E-cadherin,Invitrogen,Rat,13190,"1:1,000",IF ERG,Abcam,Rabbit,AB92513,1:2500,IF GFP,Abcam,chicken,AB13970,"1:5,000","IF,

(10) The two-faced role of RNA methyltransferase METTL3 on cellular response to cisplatin in head and neck squamous cell carcinoma in vitro modelFrontiers in oncologyJanuary 1, 2024Ostrowska K, Rawłuszko-Wieczorek AA, Ostapowicz J, Suchorska WM, Golusiński W.METTL3 (1:1,000; cat. no. PA5–121190; Invitrogen, Waltham, MA, USA) and β-tubulin (1:2,000; cat. no. PA5–16863; Invitrogen, Waltham, MA, USA) overnight at 4°C. Following the primary antibody incubation, membranes

(11) An inducible tricolor reporter mouse for simultaneous imaging of lysosomes, mitochondria and microtubulesbioRxivMay 25, 2023Vera Hutchison, Anne Lynch, Andrés Mauricio Gutierrez Gutierrez Gamez, Jichao Chentubulin,ThermoFisher,rat,MA1-80017,1:1000,IF beta actin,ThermoFisher,rabbit,MA5-32540,1:1000,WB beta tubulin,Invitrogen,rabbit,PA5-16863,1:1000,IF beta tubulin,Invitrogen,rabbit,PA5-16863,1:200,WB E-cadherin,Invitrogen,rat,13190,1:1000,IF GFP,Abcam,chicken,AB13970,1:5000,"IF,

(12) Targeting GSTP1 as Therapeutic Strategy against Lung Adenocarcinoma Stemness and Resistance to Tyrosine Kinase InhibitorsAdvanced science (Weinheim, Baden-Wurttemberg, Germany)March 1, 2023Wang SQ, Chen JJ, Jiang Y, Lei ZN, Ruan YC, Pan Y, Yam JWP, Wong MP, Xiao ZJ.(1:2000; Cell signaling #8515s), KEAP1 (1:1000; Santa Cruz #sc365626), and β‐tubulin (1:2000; Invitrogen #PA5‐16863). Intracellular Calcium Measurement Before calcium measurements, cells were washed with Margo's solution.

(13) Cytocompatible and osteoconductive silicon oxycarbide glass scaffolds 3D printed by DLP: a potential material for bone tissue regenerationFrontiers in bioengineering and biotechnologyJanuary 1, 2023Carnieri MV, Garcia DF, Voltolini R, Volpato N, Mafra M, Bernardelli EA, Stimamiglio MA, Rebelatto CK, Correa A, Berti LF, et al.permeabilized with PBS/Triton 0.1% and incubated with anti-β-tubulin rabbit polyclonal antibody (Invitrogen, PA5-16863) and anti-rabbit IgG conjugated with Alexa 488 secondary antibody (Invitrogen, A27034). The 300 nM DAPI

(14) Nanotopography modulates cytoskeletal organization and dynamics during T cell activationMolecular biology of the cellSeptember 1, 2022Wheatley BA, Rey-Suarez I, Hourwitz MJ, Kerr S, Shroff H, Fourkas JT, Upadhyaya A.using 0.3 M glycine and bovine serum albumin. For MT staining, beta tubulin antibody (Thermofisher Cat#PA5-16863) was used as primary antibody and Alexa Fluor-488 (Invitrogen, Cat# A10235) was used for secondary antibody.

(15) Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stressMolecular cellApril 21, 2022Stoneley M, Harvey RF, Mulroney TE, Mordue R, Jukes-Jones R, Cain K, Lilley KS, Sawarkar R, Willis AE.anti-FLAG,Sigma-Aldrich,Cat. #F1804; RRID: AB_262044 Rabbit polyclonal anti-tubulin,Thermo Fisher,Cat. #PA5-16863; RRID: AB_10986058 HRP conjugated goat anti-rabbit,GE Healthcare,Cat. #NA934V HRP conjugated goat

(16) Description of Osmolyte Pathways in Maturing Mdx Mice Reveals Altered Levels of Taurine and Sodium/Myo-Inositol Co-TransportersInternational journal of molecular sciencesMarch 17, 2022Merckx C, Cosemans G, Zschüntzsch J, Raedt R, Schmidt J, De Paepe B, De Bleecker JL.(0.2 µg/mL, ab 9485, Abcam, Cambridge, UK); rabbit anti-beta tubulin polyclonal antibody (0.5 µg/mL, PA5-16863, Invitrogen, Waltham, MA, US); rabbit anti-BGT-1 antibody (2 µg/mL, ab 200676, Abcam, Cambridge, UK);

(17) Uveal melanoma-associated mutations in PLCβ4 are constitutively activating and promote melanocyte proliferation and tumorigenesisScience signalingDecember 14, 2021Phan HTN, Kim NH, Wei W, Tall GG, Smrcka AV.

(18) eIF5A-Independent Role of DHPS in p21(CIP1) and Cell Fate RegulationInternational journal of molecular sciencesDecember 7, 2021Becker AE, Wu PK, Park JI.USA). β-actin 1:5000 (Sigma, St. Louis MO, USA, A1978), β-tubulin 1:5000 (Invitrogen, Waltham MA, USA, PA5-16863), eIF5AHyp 1:5000 (gift from Dr. Mirmira [45]). SuperSignal West Pico and Femto chemiluminescence kit

(19) The NOTCH3 Downstream Target HEYL Is Required for Efficient Human Airway Basal Cell DifferentiationCellsNovember 18, 2021Bodas M, Subramaniyan B, Moore AR, Metcalf JP, Ocañas SR, Freeman WM, Georgescu C, Wren JD, Walters MS.number 2586S, Cell Signaling Technologies, Danvers, MA, USA), β-tubulin (1:5000 dilution, catalog number PA5-16863, Thermo Fisher Scientific), NOTCH3/NICD3 (1:3000, catalog number 5276, Cell Signaling Technologies)

(20) The Gastroprotective Effect of Naringenin against Ethanol-Induced Gastric Ulcers in Mice through Inhibiting Oxidative and Inflammatory ResponsesInternational journal of molecular sciencesNovember 5, 2021Li WS, Lin SC, Chu CH, Chang YK, Zhang X, Lin CC, Tung YT.MA, USA), iNOS (PA3-030A, 1:2000; Thermo Fisher Scientific, Waltham, MA, USA), and β-tubulin (Cat #PA5-16863, 1:1000; Thermo Fisher Scientific, Waltham, MA, USA) at 4 °C overnight. The horseradish peroxidase (HRP)-conjugated

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beta Tubulin Antibody

beta Tubulin Antibody from Thermo Fisher Scientific

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beta Tubulin Antibody from Thermo Fisher Scientific

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