Bub3 Antibody PA5-17071 from Thermo Fisher Scientific

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WB

Western blot was performed using Anti-Bub3 Polyclonal Antibody (Product # PA5-17071) and 37 kDa band corresponding to Bub3 was observed across cell lines and tissues tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), A-431 (Lane 3), Hep G2 (Lane 4), PC-3 (Lane 5), HEK-293 (Lane 6), Jurkat (Lane 7), SK-BR-3 (Lane 8), tissue extracts of Mouse Brain (Lane 9) and Mouse Spleen (Lane 10) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). .
ICC/IF

Immunofluorescence analysis of Bub3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Bub3 Polyclonal Antibody (Product # PA5-17071) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents cells with no primary antibody to assess background. The images were captured at 60X magnification..
Bub3 Antibody

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Thermo Fisher Scientific for
Bub3 Antibody

The Bub3 Antibody from Thermo Fisher Scientific is a Rabbit Polyclonal antibody to BUB3. This antibody recognizes Homo Sapiens, Human, Mouse, Rat, Hamster, and Non-Human Primate antigen. The Bub3 Antibody has been shown to work in the following applications: Immunocytochemistry, and Western Blot.

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Bub3 Antibody

Bub3 Antibody from Thermo Fisher Scientific

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Bub3 Antibody from Thermo Fisher Scientific

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