Western blot was performed using Anti-c-Cbl polyclonal Antibody (Product # PA5-17453) and a 110 kDa band corresponding to c-Cbl was observed in Mouse testis, Rat testis, THP-1, BeWo, Daudi and SH-SY5Y but was absent in Mouse Heart and Rat heart, as reported. Tissue extracts (30 µg lysate) of Mouse testis (Lane 1), Rat testis (Lane 2), Mouse heart (Lane 3), Rat heart (Lane 4), and whole cell extracts (30 µg lysate) of THP-1 (Lane 5), BeWo (Lane 6), Daudi (Lane 7) and SH-SY5Y (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG Heavy Chain Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).