Western blot was performed using Anti-JMJD1B Polyclonal Antibody (Product # PA5-17170) and a 200 kDa band corresponding to JMJD1B was observed across all cell lines tested. Nuclear enriched extracts (50 µg lysate) of IMR-32 (Lane 1), HeLa (Lane 2), Caco-2 (Lane 3), NCCIT (Lane 4), K-562 (Lane 5) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).