Nucleolin Antibody PA5-19508 from Thermo Fisher Scientific

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WB

Western blot was performed using Anti-Nucleolin Polyclonal Antibody (Product # PA5-19508). Nucleolin is a ubiquitinylated protein for which the molecular weight is reported to be 76.6 kDa to 100 kDa, which can be observed in the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), SH-SY5Y (Lane 2), NTERA-2 cl.D1 (Lane 3), A-431 (Lane 4), PANC-1 (Lane 5), Raji (Lane 6), Mouse liver (Lane 7) and Mouse brain (Lane 8) were electrophoresed using Novex® NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
ICC/IF

Immunofluorescence analysis of Nucleolin was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Nucleolin Polyclonal Antibody (Product # PA5-19508) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear expression of Nucleolin. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Nucleolin Antibody

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Thermo Fisher Scientific for
Nucleolin Antibody

The Nucleolin Antibody from Thermo Fisher Scientific is a Rabbit Polyclonal antibody to NCL, and nucleolin. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The Nucleolin Antibody has been shown to work in the following applications: Immunocytochemistry, Immunohistochemistry, Immunohistochemistry - fixed, Immunohistochemistry - frozen, Immunoprecipitation, and Western Blot.

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Nucleolin Antibody

Nucleolin Antibody from Thermo Fisher Scientific

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Nucleolin Antibody from Thermo Fisher Scientific

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