Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1) and MKN45 (Lane 2). The blots were probed with Anti-Alpha Tubulin Rat Monoclonal Antibody (Product # MA1-80017, 1 µg/mL) and detected by chemiluminescence of alkaline phosphatase (AP) using Rabbit anti-Rat IgG Secondary Antibody, AP (Product # PA1-28787) at dilutions 1:2,000 (Fig. 1) and 1:5,000 (Fig. 2). A 52 kDa band corresponding to Alpha Tubulin was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection of alkaline phosphatase (AP) was performed using Novex® AP Chemiluminescent Substrate (CDP-Star®) (Product # WP20002) with Novex® AP Chemiluminescent Substrate Enhancer (Nitro Block II™) (Product # WP20003).