Fig 2: (a) Endothelial cell expression of total eNOS in response to untreated cells (n = 4) and treatment with microvesicles from non‐smokers (n = 9) and electronic (e‐)cigarette smokers (n = 10) and EI (n = 10). (b) Endothelial cell expression of total p‐eNOS (Ser1177) in response to untreated cells (n = 4) and treatment with microvesicles from non‐smokers (n = 9) and e‐cigarette smokers (n = 10) and EI (n = 10). (c) Endothelial cell expression of total p‐eNOS (Thr495) in response to untreated cells (n = 4) and treatment with microvesicles from non‐smokers (n = 9) and e‐cigarette smokers (n = 10) and EI (n = 10). (d) Endothelial cell expression of total NO production in response to untreated cells (n = 4) and treatment with microvesicles from non‐smokers (n = 9) and e‐cigarette smokers (n = 10) and EI (n = 10). The mean value (black line) and SD (grey brackets) are denoted for each group. Abbreviation: AU, arbitrary units; EI, endocytosis inhibitors; eNOS, endothelial nitric oxide synthase.

Fig 3: (a) Endothelial cell expression of total eNOS in response to untreated cells (n = 4) and treatment with microvesicles from non‐smokers (n = 9) and electronic (e‐)cigarette smokers (n = 10) and EI (n = 10). (b) Endothelial cell expression of total p‐eNOS (Ser1177) in response to untreated cells (n = 4) and treatment with microvesicles from non‐smokers (n = 9) and e‐cigarette smokers (n = 10) and EI (n = 10). (c) Endothelial cell expression of total p‐eNOS (Thr495) in response to untreated cells (n = 4) and treatment with microvesicles from non‐smokers (n = 9) and e‐cigarette smokers (n = 10) and EI (n = 10). (d) Endothelial cell expression of total NO production in response to untreated cells (n = 4) and treatment with microvesicles from non‐smokers (n = 9) and e‐cigarette smokers (n = 10) and EI (n = 10). The mean value (black line) and SD (grey brackets) are denoted for each group. Abbreviation: AU, arbitrary units; EI, endocytosis inhibitors; eNOS, endothelial nitric oxide synthase.