BCR Antibody PA5-17709 from Thermo Fisher Scientific

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WB

Western blot was performed using Anti-BCR Polyclonal Antibody (Product # PA5-17709) and a 160 kDa band corresponding to BCR was observed across cell lines and tissue tested. Whole cell extracts (30 µg lysate) of Ramos (Lane 1), K-562 (Lane 2), MOLT-4 (Lane 3), A-431 (Lane 4), MCF7 (Lane 5), Hep G2 (Lane 6), Jurkat (Lane 7), PC-12 (Lane 8) and tissue extract (30 µg lysate) of Mouse Kidney (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by XCell SureLock™ Mini-Cell and XCell II™ Blot Module (Product # EI0002). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
ICC/IF

Immunofluorescence analysis of BCR was performed using K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with BCR Polyclonal Antibody (Product # PA5-17709) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification..
BCR Antibody

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Thermo Fisher Scientific for
BCR Antibody

The BCR Antibody from Thermo Fisher Scientific is a Rabbit Polyclonal antibody to BCR, and BCR activator of RhoGEF and GTPase. This antibody recognizes Homo Sapiens, Human, Mouse, Rat, and Porcine antigen. The BCR Antibody has been shown to work in the following applications: Immunocytochemistry, and Western Blot.

WB

ICC/IF

BCR Antibody

BCR Antibody from Thermo Fisher Scientific

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BCR Antibody from Thermo Fisher Scientific

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