LDHA Antibody PA5-19754 from Thermo Fisher Scientific

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WB

Western blot was performed using Anti-LDHA Polyclonal Antibody(Product # PA5-19754) and a 35 kDa band corresponding to LDHA was observed across cell lines and tissue extracts tested. A non-specific band around 50 kDa has also been picked up in tissues extracts. Whole Cell Extract-WCL (30 µg lysate) of PC-3 (Lane 1), DU 145 (Lane 2), PANC-1 (Lane 3), MCF7 (Lane 4), MDA-MB-231 (Lane 5) and tissue extracts of Mouse Skeletal Muscle (Lane 6) and Rat Skeletal Muscle (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
ICC/IF

Immunofluorescence analysis of LDHA was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with LDHA Polyclonal Antibody (Product # PA5-19754) at 2.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
LDHA Antibody

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Thermo Fisher Scientific for
LDHA Antibody

The LDHA Antibody from Thermo Fisher Scientific is a Rabbit Polyclonal antibody to Lactate Dehydrogenase A, and LDHA. This antibody recognizes Homo Sapiens, and Human antigen. The LDHA Antibody has been shown to work in the following applications: Immunocytochemistry, Immunohistochemistry, Immunohistochemistry - fixed, Immunoprecipitation, and Western Blot.

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LDHA Antibody

LDHA Antibody from Thermo Fisher Scientific

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LDHA Antibody from Thermo Fisher Scientific

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