Western blot was performed using Anti-CTCF Polyclonal Antibody (Product # PA5-17143) and a 110 kDa band corresponding to CTCF was observed across all the cell lines tested and as reported, decrease in the expression of CTCF was observed in MCF7 treated with Trichostatin A. Modified whole cell extracts (1% SDS) (30 µg lysate) of Fig (a) HeLa (Lane 1), PC-12 (Lane 2), HEK-293 (Lane 3), NIH/3T3 (Lane 4), HCT 116 (Lane 5), Jurkat (Lane 6), RAW 264.7 (Lane 7), Hep G2 (Lane 8); Fig (b) MCF7 (Lane 1) and MCF7 treated with Trichostatin A (10uM for 24hr) (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).