Fig 2: Endotoxemia induced a distinct temporal and substrate specificity for splenic SUMO isoforms. Western-blots (top) and densitometric analyses (bottom) of splenic SUMOylation at the indicated post-LPS time points. Bacterial endotoxin induced SUMOylation-1 of (A) p150, (B) p100, (C) p250, and (D) SUMOylation-2/3 of p70 and p260 (smear). Comparative densitometric analyses of (E) SUMO1 and (F) SUMO2/3 at the indicated post-LPS time-points. ß-actin Western-blots (bottom panels) were used as an internal control for protein loading. were used as an internal control for protein loading. Western-blots represent experiments repeated at least twice on different days and graphs show mean ± SEM of fold of induction (FI). *P<0.05 vs Control (n=3/group, two-way ANOVA with Tukey’s post hoc test).