Fig 1: Analysis of protein expression by quantitative immunoblots for a the transcriptional immune modulator STAT1, b the cytosolic RNA sensor IFIT3, as well as the RLR pathway components c DDX58, d TRIM25, and e ISG15, in MEFs and brain from 3- versus 11–19-month-old mice. Immunoblots were normalized to ACTB or GAPDH. Data are presented as mean ± SEM. In bar graphs, WT is shown as checked, while ClpP−/− as plain colored. Statistical significances are illustrated by symbols: * or # p < 0.05; ** or §§ p < 0.01; *** or §§§ p < 0.001; **** or §§§§ p < 0.0001; not significant (ns) p > 0.05. Asterisks portray significant differences between WT and ClpP−/− MEFs, hashtags show significant effects in the brain between 3-month-old WT and ClpP−/− mice, and the section sign visualizes significant differences in the brain from 11–19-month-old WT versus ClpP−/− mice. n. s. b., no specific bands. WT MEF: n= 3–5; ClpP−/− MEF: n= 3–5; WT brain 3 months: n= 3; ClpP−/− brain 3 months: n= 3; WT brain 11–19 months: n= 5; ClpP−/− brain 11–19 months: n= 6