Immunofluorescent staining of SH-SY5Y cells using Product # PA5-19588, anti-CAPS1 antibody. The cells were fixed with methanol (100%) for 5 minutes, permabilised with TBS-T (20mins), BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% PBS-Tween for 1 hour and exposed to the primary antibody at a concentration of 1 µg/mL for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).