HA Tag Antibody 26183 from Thermo Fisher Scientific

Product Specs
ItemHA Tag Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number26183
HostMouse
ApplicationsImmunocytochemistry, Immunoprecipitation, Western Blot
Clone2-2.2.14
FormatPBS with 0.05% sodium azide; pH 7.4
TargetHA Tag
IsotypeIgG1
Brand/TrademarkABR (now Thermo Scientific Pierce Antibodies)
Quantity100 µg
ReactivityTag
ClonalityMonoclonal
Concentration1 mg/mL
ImmunogenHA peptide YPYDVPDYA derivitized to ovalbumin
Storage Temperature-20° C, Avoid Freeze/Thaw Cycles
PurityProtein G
Conjugate/TagUnconjugated
Antibody TypePrimary
Storage-20° C, Avoid Freeze/Thaw Cycles

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Site-specific phosphorylation of ZYG-1 regulates ZYG-1 stability and centrosome numberiScienceDecember 15, 2023Jeffrey C. Medley, Rachel N. Yim, Joseph DiPanni, Brandon Sebou, Blake Shaffou, Evan Cramer, Colin Wu, Megan Kabara, Mi Hye SongT9026), α-ZYG-1,57 α-SAS-4,27 α-Myc (ThermoFisher, PA1-981), α-V5 (MBL, M167-3), α-HA (ThermoFisher, 26183) and α-Ollas (ThermoFisher, MA5-16125). Alexa Fluor 488 and 568 secondary antibodies (ThermoFisher,

(2) Translational control by Trypanosoma brucei DRBD18 contributes to the maintenance of the procyclic stateRNADecember 1, 2023Martin Ciganda, José Sotelo-Silveira, Ashutosh P. Dubey, Parul Pandey, Joseph T. Smith, Shichen Shen, Jun Qu, Pablo Smircich, Laurie K. Read(Ciganda et al. 2012), or mouse monoclonal antibody against hemagglutinin (Thermo Scientific, cat # 26183) (1:5000 dilution). Blots were washed and incubated with either goat anti-rabbit HRP or goat anti-mouse

(3) A protease and a lipoprotein jointly modulate the conserved ExoR-ExoS-ChvI signaling pathway critical in Sinorhizobium meliloti for symbiosis with legume hostsPLOS GeneticsOctober 23, 2023Julian A. Bustamante, Josue S. Ceron, Ivan Thomas Gao, Hector A. Ramirez, Milo V. Aviles, Demsin Bet Adam, Jason R. Brice, Rodrigo A. Cuellar, Eva Dockery, Miguel Karlo Jabagat, et al.anti-V5 (Invitrogen R960-25; AB_2556564) was used at 1:2500 dilution, anti-HA [clone 2–2.2.14] (Thermo 26183; AB_10978021) used at 1:1000 to 1:5000 dilution, and antibody to E. coli ATPase B [7E3F2] (Abcam ab110280)

(4) A DRP-like pseudoenzyme coordinates with MICOS to promote cristae architecturebioRxivOctober 11, 2023Abhishek Kumar, Mehmet Oguz Gok, Kailey N. Nguyen, Michael L. Reese, Jeremy G. Wideman, Sergio A. Muñoz-Gómez, Jonathan R. Friedmanprimary antibodies (FLAG (F1804; Sigma-Aldrich), rabbit α-GAPDH (10494-1-AP; Proteintech), mouse α-HA (26183; 1:2000; Thermo Fisher Scientific), rabbit α-GFP (ab290; Abcam). To detect Mic19-3xHA, goat anti-mouse

(5) CXCR3-CXCL11 signaling restricts angiogenesis and promotes pericyte recruitmentbioRxivSeptember 22, 2023Megan E. Goeckel, Jihui Lee, Allison Levitas, Sarah Colijn, Geonyoung Mun, Zarek Burton, Bharadwaj Chintalapati, Ying Yin, Javier Abello, Amber Stratmanincubated with Dynabeads Protein G (#10007D, Invitrogen) coated with 3 μg of HA-Tag monoclonal antibody (#26183, ThermoFisher Scientific) at 4°C for overnight. Unbound materials were washed off using high salt homogenization

(6) A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtiiThe Plant CellSeptember 1, 2023Chun Sing Lau, Adam Dowle, Gavin H Thomas, Philipp Girr, Luke C M MackinderStreptavidin Dylight-488 conjugate (1:4,000, Fisher Scientific #21832); anti-HA (1:1,000, Fisher Scientific 26183); anti-Flag (1:1,000, Sigma #F1804); and antitubulin (1:2,000, Sigma #T6074). Biotin labeling and streptavidin

(7) The AT-hook is an evolutionarily conserved auto-regulatory domain of SWI/SNF required for cell lineage primingNature CommunicationsAugust 4, 2023Dhurjhoti Saha, Solomon Hailu, Arjan Hada, Junwoo Lee, Jie Luo, Jeff A. Ranish, Yuan-chi Lin, Kyle Feola, Jim Persinger, Abhinav Jain, et al.primary antibodies anti-Brg1/Smarca4 (abcam; ab110641; dilution 1:1000), anti-HA (Invitrogen; Cat #26183; dilution 1:1000), anti-Tubulin (ThermoFisher; A11126; dilution 1:1000), and anti-Gapdh (CST; 2118;

(8) Extensive characterization of a Williams syndrome murine model shows Gtf2ird1-mediated rescue of select sensorimotor tasks, but no effect on enhanced social behaviorGenes, brain, and behaviorAugust 1, 2023Nygaard KR, Maloney SE, Swift RG, McCullough KB, Wagner RE, Fass SB, Garbett K, Mirnics K, Veenstra-VanderWeele J, Dougherty JD.verified by a Western blot using an antibody to the HA tag (Mouse anti‐HA, 1:1000, Thermo Scientific, 26183, not shown). As the HA tag is included only on some transcripts of Gtf2ird1 because of alternative splicing

(9) The Role of the Tyrosine-Based Sorting Signals of the ORF3a Protein of SARS-CoV-2 on Intracellular Trafficking, Autophagy, and ApoptosisbioRxivJuly 27, 2023Wyatt Henke, Maria Kalamvoki, Edward B Stephensand immunoblot analysis using a mouse monoclonal antibody directed against the HA-tag (Thermo-Fisher, catalog # 26183; antibody 2-2.2.14). Other plasmids that expressed organelle markers tagged with fluorescent proteins

(10) A proximity labeling strategy enables proteomic analysis of inter-organelle membrane contactsiScienceJuly 21, 2023Zhou M, Kong B, Zhang X, Xiao K, Lu J, Li W, Li M, Li Z, Ji W, Hou J, et al.AB_471093 HRP conjugated streptavidin,Pierce,Cat#21130 HA Tag Monoclonal Antibody,Invitrogen,Cat#26183; RRID: AB_10978021 SQLE Polyclonal antibody,Proteintech,Cat#12544-1-AP; RRID: AB_2195888 COXIV Polyclonal

(11) A cooperative network at the nuclear envelope counteracts LINC-mediated forces during oogenesis in C. elegansScience AdvancesJuly 14, 2023Chenshu Liu, Rachel Rex, Zoe Lung, John S. Wang, Fan Wu, Hyung Jun Kim, Liangyu Zhang, Lydia L. Sohn, Abby F. Dernburgantibodies were used: anti-V5 (mouse, P/N 46-0705, Invitrogen), anti-hemagglutinin (HA) (mouse monoclonal, #26183, Invitrogen), anti-GFP (1:400; mouse monoclonal, Roche), anti–SYP-1 [1:400; goat, affinity purified

(12) NeuroMabSeq: high volume acquisition, processing, and curation of hybridoma sequences and their use in generating recombinant monoclonal antibodies and scFvs for neuroscience researchbioRxivJuly 5, 2023Keith G. Mitchell, Belvin Gong, Samuel S. Hunter, Diana Burkart-Waco, Clara E. Gavira-O’Neill, Kayla M. Templeton, Madeline E. Goethel, Malgorzata Bzymek, Leah M. MacNiven, Karl D. Murray, et al.For scFvs, sections were incubated for 1 h in anti-HA 2-2.2.14 IgG1 mouse mAb (ThermoFisher Catalog# 26183; RRID:AB_10978021) diluted 1:2000 and following 3 x 10 min washes in 0.1 M PB, sections were incubated

(13) Direct fluorescent labeling of NF186 and NaV1.6 in living primary neurons using bioorthogonal click chemistryJournal of Cell ScienceJune 15, 2023Nevena Stajković, Yuanyuan Liu, Aleksandra Arsić, Ning Meng, Hang Lyu, Nan Zhang, Dirk Grimm, Holger Lerche, Ivana Nikić-Spiegelwere as follows: mouse anti-HA tag (2-2.2.14) antibody (1:1000; Thermo Fisher Scientific, cat. no. 26183) was used for click labeling of NF186–HA in ND7/23 cell line; rabbit anti-HA tag (C29F4) monoclonal

(14) Engineered Yeast Displaying Specific Norovirus-Binding Nanobodies for the Concentration and Detection of Human Norovirus in Food MatrixJournal of Agricultural and Food ChemistryJune 7, 2023Xue Zhao, Mahbubur Rahman, Zhiyuan Xu, Tom Kasputis, Yawen He, Lijuan Yuan, R. Clay Wright, Juhong ChenThermo Fisher Scientific (Rockford, IL). Antibodies, including Anti-HA Tag Monoclonal Antibody with Dylight488 (26183) and Alexa Fluor 488 goat antimouse IgG (H+L) (A11001), were purchased from Invitrogen (Eugene, OR).

(15) Site-Specific Phosphorylation of ZYG-1 Regulates ZYG-1 Stability and Centrosome NumberbioRxivJune 3, 2023Jeffrey C. Medley, Nahyun Yim, Joseph DiPanni, Brandon Sebou, Blake Shaffou, Evan Cramer, Colin Wu, Megan Kabara, Mi Hye SongT9026), α-ZYG-156, α-SAS-427, α-Myc (ThermoFisher, PA1–981), α-V5 (MBL, M167–3), α-HA (ThermoFisher, 26183) and α-Ollas (ThermoFisher, MA5–16125). Alexa Fluor 488 and 568 secondary antibodies (ThermoFisher,

(16) Synthetic virology approaches to improve the safety and efficacy of oncolytic virus therapiesNature CommunicationsMay 26, 2023Taha Azad, Reza Rezaei, Ragunath Singaravelu, Adrian Pelin, Stephen Boulton, Julia Petryk, Kemal Alper Onsu, Nikolas T. Martin, Victoria Hoskin, Mina Ghahremani, et al.primary antibody. The following antibodies SARS-CoV-2 Spike (GeneTex, 1A9), HA (Thermo Fisher Scientific, 26183), VV (Abcam, ab117453), and GADPH (Cell Signaling, 2118L) antibodies, which were used at a dilution

(17) Computational design of dynamic receptor—peptide signaling complexes applied to chemotaxisNature CommunicationsMay 19, 2023Robert E. Jefferson, Aurélien Oggier, Andreas Füglistaler, Nicolas Camviel, Mahdi Hijazi, Ana Rico Villarreal, Caroline Arber, Patrick BarthBSA for 45 min. After that, 45 min incubations, first with an anti-HA antibody (Thermofisher, ref: 26183) at a dilution of 1:500 followed by a second anti-mouse IgG antibody (CST, ref: 7076S) at 1:2000 dilution,

(18) Centromere proteins are asymmetrically distributed between newly divided germline stem and daughter cells and maintain a balanced niche in Drosophila malesMolecular Biology of the CellMay 1, 2023Antje M. Kochendoerfer, Rachel S. Keegan, Elaine M. Dunleavyanti-BAM (DSHB; 1:50), mouse anti-FIBRILLARIN (Abcam; ab5821; 1:500), mouse anti-HA (ThermoFisher; 26183; 1:500), mouse anti-gamma-tubulin (Abcam; ab44928; 1:500), mouse anti-FASCILLIN III (DSHB 7G10; 1:200),

(19) Cold temperature extends longevity and prevents disease-related protein aggregation through PA28γ-induced proteasomesNat AgingMay 1, 2023Lee HJ, Alirzayeva H, Koyuncu S, Rueber A, Noormohammadi A, Vilchez D.(Proteintech, 14907-1-AP, 1:200), Mouse anti-FUS (Abcam, ab154141, 1:200), and Mouse anti-HA tag (ThermoFisher, 26183, clone 2-2.2.14, 1:200)). Then, cells were washed with PBS and incubated with secondary antibody (Alexa

(20) Pain-causing stinging nettle toxins target TMEM233 to modulate NaV1.7 functionNature CommunicationsApril 28, 2023Sina Jami, Jennifer R. Deuis, Tabea Klasfauseweh, Xiaoyang Cheng, Sergey Kurdyukov, Felicity Chung, Andrei L. Okorokov, Shengnan Li, Jiangtao Zhang, Ben Cristofori-Armstrong, et al.cell surface exposure of N- or C-terminal tagged HA-TMEM233, 0.5 µg/mL of HA Tag monoclonal antibody (#26183, ThermoFisher Scientific) diluted in media was incubated with cells for 1 h inside a 5% CO2 tissue culture

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(1)

2/12/2024Anti-HA Antibody From Invitrogen for Immunofluorescence Parijat SarkarI used HA Tag Monoclonal Antibody to label 3X-HA tagged protein expressed in 293T cells. The HA-tag is small compared to conventional fluorescent proteins and therefore is less likely to interfere ... Read Review
(1)

4/15/2021Anti-HA Antibody Works Well Xiaoguang LiThere is one band sometimes several bands at expected molecular weight. The signal of this antibody is not strong. Background could be detected.
(1)

4/3/2018Excellent Antibody To Detect HA Tag In Western BlottingDr. Elena DeryuginaTo assist the detection of de novo expressed proteins, different tags could be used in the protein constructs that are transfected or infected into host cells. Expression of specifically tagged ... Read Review

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HA Tag Antibody

HA Tag Antibody from Thermo Fisher Scientific

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HA Tag Antibody from Thermo Fisher Scientific

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