RNF168 Antibody AF7217 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRNF168 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF7217
TargetRNF168
IsotypeIgG
ReactivityHuman,Mouse
HostSheep
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
NCBI Gene AliaseshRNF168, RIDL, 3110001H15Rik
ApplicationsImmunoprecipitation,Western Blot,Immunohistochemistry-Frozen,Immunocytochemistry
NCBI Full Gene Namering finger protein 168
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenE. coli-derived recombinant mouse RNF168, Asn423-Arg565, Accession # Q80XJ2
ConcentrationLYOPH
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
PurityAntigen Affinity-purified

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects recombinant mouse RNF168 and recombinant human RNF168 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signalingMolecular cellMay 18, 2023Longarini EJ, Dauben H, Locatelli C, Wondisford AR, Smith R, Muench C, Kolvenbach A, Lynskey ML, Pope A, Bonfiglio JJ, et al.Monoclonal Antibody (JL-8),Takara,Cat# 632381; RRID:AB_2313808 Anti-RNF168 antibody,Novus Biologicals,Cat# AF7217; RRID:AB_10971653 Anti-RIF1 antibody,Abcam,Cat# ab229656 Anti-phospho-Histone H2A.X (Ser139) antibody,Millipore,Cat#

(2) RNF168-mediated localization of BARD1 recruits the BRCA1-PALB2 complex to DNA damageNature communicationsAugust 18, 2021Krais JJ, Wang Y, Patel P, Basu J, Bernhardy AJ, Johnson N.The following primary antibodies were used: HA (Cell Signaling, 2367; 1:1000), RNF168 (R&D Systems, AF7217; 1:1000), RNF168 (Millipore, ABE367; 1:1000), BRCA1 (Millipore, OP92; 1:500), BARD1 (Bethyl Laboratories,

(3) RNF168 regulates R-loop resolution and genomic stability in BRCA1/2-deficient tumorsThe Journal of clinical investigationFebruary 1, 2021Patel PS, Abraham KJ, Guturi KKN, Halaby MJ, Khan Z, Palomero L, Ho B, Duan S, St-Germain J, Algouneh A, et al.The expression of RNF168 was examined by Western blotting using anti-RNF168 antibody (R&D Systems, AF7217). The expression of BRCA1 was examined by Western blotting using antiBRCA1 antibody (Abcam, 16780).

(4) RNF168-Mediated Ubiquitin Signaling Inhibits the Viability of BRCA1-Null CancersCancer researchJuly 1, 2020Krais JJ, Wang Y, Bernhardy AJ, Clausen E, Miller JA, Cai KQ, Scott CL, Johnson N.catalog# sc-271462 ), RNF168 (EMD Millipore, catalog# ABE367 and #06-1130-I, R&D Systems, catalog# AF7217), Tubulin (Cell Signaling, catalog# 2148), GFP (Santa Cruz Biotechnology, catalog# sc-9996), RFP (ChromoTek,

(5) 53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous RecombinationMolecular cellJanuary 2, 2020Callen E, Zong D, Wu W, Wong N, Stanlie A, Ishikawa M, Pavani R, Dumitrache LC, Byrum AK, Mendez-Dorantes C, et al.polyclonal antiRPA32/RPA2,Abcam,Cat# ab76420; RRID:AB_1524336 Sheep polyclonal anti-RNF168,R&D System,Cat# AF7217; RRID:AB_10971653 Mouse monoclonal anti-RAD18,Millipore,Cat# ABE1377 Mouse monoclonal anti-FLAG (M2),Sigma-Aldrich,Cat#

(6) RNF168 and USP10 regulate topoisomerase IIα function via opposing effects on its ubiquitylationNature communicationsAugust 25, 2016Guturi KK, Bohgaki M, Bohgaki T, Srikumar T, Ng D, Kumareswaran R, El Ghamrasni S, Jeon J, Patel P, Eldin MS, et al.components. After washing with 3% BSA in PBS, the sheep polyclonal against RNF168 (1:2,000; R&D systems cat# AF7217) and the rabbit polyclonal against TOP2α (Abcam) were detected using their respective secondary antibodies.

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RNF168 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (6)

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RNF168 Antibody from R&D Systems, a Bio-Techne Brand

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