Fig 1: OTX2 O-GlcNAc-site mutants lose interactions with the chaperonin CCT5(A) Volcano plot of proteins commonly lost or gained as OTX2 interactors in WT and mutant constructs (n = 2).(B) STRING network analysis of the 19 common proteins dislodged from OTX2 when one or all O-GlcNAc sites found were mutated to alanine.(C) Interactome analysis of the TriC complex.(D) HeLa cells transfected with OTX2 WT or mutant 3A. OTX2 was pulled down and analyzed by Western Blot. Optical density quantification of Western Blot bands. One-way ANOVA, p < 0.05 (∗) (n = 2).(E) HeLa cells transfected with OTX2 WT or empty vector EV-OSF, lysates were crosslinked with DSP. OTX2 was pulled down by Strep-Tactin beads from both soluble and pellet fractions and analyzed by Western Blot. Data are represented as mean ± SEM in panels presenting pooled data.See also Figures S5G, S5H and Table S3.