Fig 1: Association of different proteins with transcriptional splicing as assessed by NET-prism. (a) Heatmaps and metaplots assessing polymerase pausing for total Pol II and Splicing factor 1 (Sf1) over exon boundaries (n = 5,550). Solid lines indicate the mean values, whereas the shading represents the 95% confidence interval. (b) Boxplots measuring Pol II coverage over exons (n = 41,356) and introns (n = 199,172) for each NET-seq/prism library. First and last exons are removed from the analysis. (c) RNA Pol II interrogation of all NET-seq/prism libraries over a single gene (Actb). Exons are highlighted in purple.