Fig 1: Knockdown of TOP3α, BLM, ABRAXAS2, RAD54L2, and ZZZ3.(A) Control (open circles) or TOP3α siRNA (closed circles) was transfected into U2OS cells. After 48 h, protein depletion was examined by immunoblot analysis, probing with the antibody against TOP3α or GAPDH (as a loading control). Corresponds to Fig. 3B. (B) Control (open circles) or the indicated siRNAs (closed circles) were transfected into U2OS cells. After 48 h, protein depletion was examined by immunoblot analysis, probing with the indicated antibodies. Anti-tubulin blots are included as loading controls. Small circles mark putative RAD54L2 and ZZZ3 polypeptides. Corresponds to Fig. 3C. (C) Control (open circles), the indicated siRNAs (closed circles), or the indicated siRNAs and the I-SceI expression plasmid (closed squares) were transfected into U2OS DR-GFP cells. After 48 h, protein depletion was examined by immunoblot analysis, probing with the indicated antibodies. Anti-tubulin blots are included as loading controls. Corresponds to Fig. 3D. Source data are available online for this figure.