Fig 1: Impact of A-C linker depletion on other centriolar elements.a Expanded daughter centrioles in longitudinal view stained for α/β tubulin (magenta) and CEP135 (green), under indicated siRNAs, n = 3. Scale bars = 100 nm. b Normalized CEP135 intensity profiles along the proximal to the distal part of the centriole length (nm) in the siCTRL (black line), siCCDC77 (green line), siWDR67 (cyan line), and siMIIP (magenta line). SDs as dashed lines. Right part: model highlighting CEP135 localization domains, with signal: 1, corresponding to the distal dot localization; 2, corresponding to the proximal localization, and 3 corresponding to the sub-proximal localization. c Normalized relative intensity of CEP135 signal from 180 to 380 nm in length from the graph (b) in the different siRNA conditions in expanded centrioles. siCTRL, N = 13, n = 3; siCCDC77, N = 11, n = 3; siWDR67, N = 9, n = 3; siMIIP, N = 8, n = 3. Kruskal-Wallis test (Dunn’s multiple comparison). d Coverage of CEP135, expressed as a percentage of the tubulin length in the indicated siRNA conditions on daughter centrioles. One-way ANOVA, two-sided (Dunn’s multiple comparison), *p = 0.0239. e CEP135 length of daughter centrioles in the indicated siRNA conditions. d, e siCTRL, N = 89, n = 5; siCCDC77, N = 69, n = 3; siWDR67, N = 67, n = 4; siMIIP, N = 25, n = 3. Kruskal-Wallis test (Dunn’s multiple comparison), **p = 0.0095. f As in a, with CEP44 (green). Right part: model of CEP44 localization. Scale bars = 100 nm. g, h As is d, e, with CEP44. siCTRL, N = 67, n = 8; siCCDC77, N = 42, n = 5; siWDR67, N = 46, n = 5; siMIIP, N = 57, n = 4. Kruskal-Wallis test (Dunn’s multiple comparison). i As in a, with SPICE (cyan). Right part: model of SPICE localization. Scale bars = 100 nm. j, k As in d, e, with SPICE. siCTRL, N = 130, n = 7; siCCDC77, N = 100, n = 4; siWDR67, N = 42, n = 4; siMIIP, N = 40, n = 4. One-way ANOVA, two-sided (j), Kruskal-Wallis test (Dunn’s multiple comparison) (k), *p = 0.02. l As in a, with POC (green). Right part: model of POC5 localization. Scale bars = 100 nm. m, n As in d, e, with POC5. siCTRL, N = 67, n = 6; siCCDC77, N = 31, n = 3; siWDR67, N = 36, n = 5; siMIIP, N = 37, n = 3. One-way ANOVA, two-sided (m, *p = 0.0192, **p = 0.002), Kruskal-Wallis test (Dunn’s multiple comparison) (n, *p = 0.011, **p = 0.0011). Expanded centrioles treated with siCTRL (n), siPOC5 (o), or siWDR67/siPOC5 (p) stained for α/β tubulin (magenta) and POC5 (green) or WDR67 (cyan). Scale bars = 200 nm. White triangle: broken centrioles with distal attachment; white star: pieces of centriole; white arrowhead: microtubule blades. Percentage of cells with broken centrioles (r, siCTRL, N = 68, n = 3; siPOC5, N = 79, n = 3; siWDR67/siPOC5, N = 127, n = 3, One-way ANOVA, two-sided – Dunn’s multiple comparison, **p = 0.0025) or with specific break types (s, siCTRL, N = 68, n = 3; siPOC5, N = 20, n =_3; siWDR67/siPOC5, N = 103, n = 3, Two-way ANOVA, two-sided – Dunn’s multiple comparison) under each siRNA. t As in o–q, stained for C2CD3 (green). Right part: model of broken centriole with distal attachment, n = 3. Scale bars = 200 nm. Dots show samples or replicates, and bars represent mean ± SD. N: number of cells, n: number of independent experiments. ****p < 0.0001. Source data for this figure are provided as a Source Data file.