RUNX2 (D1H7) Rabbit mAb 8486S from Cell Signaling Technology

Product Specs
ItemRUNX2 (D1H7) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number8486S
Quantity100 µl
ApplicationsW, IP, ChIP
ReactivityH, M, R, Mk
TargetAML3
HostRabbit
NCBI Gene AliasesAML3, CBF-alpha-1, CBFA1, CCD, CCD1, CLCD, OSF-2, OSF2, PEA2aA, PEBP2aA
ClonalityMonoclonal
NCBI Full Gene NameRUNX family transcription factor 2
Research AreaDevelopmental Biology
IsotypeIgG
CloneD1H7
Molecular Weight55-62

Questions and Answers

Can this RUNX2 antibody be used for studying osteogenic differentiation in mouse embryonic stem cells?

Yes, the RUNX2 (D1H7) Rabbit mAb has been shown to work in applications related to osteogenic differentiation. For instance, in a study, it was utilized to investigate the role of c-Src in inhibiting the transcriptional activity of RUNX2 during osteogenic differentiation of mouse embryonic stem cells and preosteoblasts ().

Is this antibody effective for immunoprecipitation assays?

The RUNX2 (D1H7) Rabbit mAb has been successfully used in immunoprecipitation assays, as demonstrated in various studies where it was employed to pull down RUNX2 protein complexes for further analysis (, ).

What species can this antibody be used with?

This antibody is reactive with several species, including human, mouse, rat, and non-human primates, making it versatile for various experimental models in developmental biology research.

Has this antibody been cited in studies related to cancer research?

Yes, the RUNX2 (D1H7) Rabbit mAb has been referenced in cancer research, particularly in studies exploring its role in the survival of p53 defective cancer cells, where it was found to mediate survival signals through the MYC transcription factor ().

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Identification of mitochondria-related genes in calcific aortic valve disease by integrated analysis of single-cell and bulk transcriptomic atlasesJ Physiol BiochemApril 10, 2026Ziling Mai, Huijun Du, Yuhan Chen, Yaner Lu, Bingchen Liu, Deqiang Sun(Abcam, #ab131059, 1:1,000), ALP (R&D Systems, #MAB29092, 1:1,000), RUNX2 (Cell Signaling Technology, #8486S, 1:1,000), and GAPDH (Huabio, #ET1702-66, 1:10,000). Immunofluorescence staining Frozen sections of

(2) Co-incorporation of Phosphorus and zinc into wollastonite ceramic granules synergically facilitating thin-walled structures regenerationScientific ReportsMarch 16, 2026Lingling Dong, Yongzheng Li, Yuting Feng, Xijuan Wang, Yonghong Liu, Xiurong Ke, Lijun Yu, Lijie Fan(clone D80F2; Cell Signaling Technology, #5731), anti-RUNX2 (clone D1H7; Cell Signaling Technology, #8486), anti-β-catenin (Cell Signaling Technology, #8480), anti-SP7 (Abcam, ab209484), and anti-β-Actin (Sigma-Aldrich,

(3) Orphan Nuclear Receptor NR4A1 Promotes Proliferation and Osteogenic Differentiation of Valvular Interstitial Cells through Activation of CCND2International Journal of Biological SciencesFebruary 26, 2026Qiang Shen, Chao Zhang, Chen Jiang, Zongtao Liu, Lin Fan, Hanshen Luo, Xingyu Qian, Zhengfeng Fan, Dingyi Yao, Nianguo Dong, et al.of all antibodies and reagents used in this study: runt-related transcription factor 2 (RUNX2) (CST, 8486, dilution ratio of 1:1000 for WB), RUNX2 (Santa Cruz, sc-390715, dilution ratio of 1:100 for Immunofluorescence

(4) β2-adrenoceptor signaling in chondrocytes regulates long bone growth, bone homeostasis, and fracture healing in miceJBMR PlusFebruary 14, 2026Melanie Rebecca Kuhn, Melanie Haffner-Luntzer, Sandra Dieterich, Meike Sachs, Gundula Rösch, Zsuzsa Jenei-Lanzl, Stefan Oskar Reber, Anita Ignatius, Miriam Eva Angelica Tschaffon-Mülleranti-mouse Col10, 1:200, #ABIN1077945, Antibodies online), Runx2+ (rabbit anti-mouse Runx2, 1:50, #8486, Cell Signaling Technology), Proliferating Cell Nuclear Antigen (PCNA+) (rabbit anti-mouse PCNA, 1:100,

(5) NLRP12 Alleviated Periodontal Destruction via Suppressing Nuclear Factor Kappa-B Signalling PathwayInt Dent JFebruary 5, 2026Shuangshuang Xu, Weiping Wang, Yuhui Sun, Juefei Wu, Wenting Zhang, Gang Dingminutes, the membranes were incubated with primary antibodies, including COL1 (#12256; CST), RUNX2 (#8486; CST), NLRP12 (#DF14960; Affinity Biosciences), phospho-p65 (p-p65) (#3033; CST), p65 (#8242; CST),

(6) Construction of a Three-Dimensional Calcific Aortic Valve Disease Model Using Human iPSC-Derived Valvular Interstitial CellsStem Cell Reviews and ReportsFebruary 1, 2026Ruikang Guo, Zhen Qi, Ping Qiu, Dogukan Mizrak, Bo Yangalbumin, membranes were incubated overnight at 4 °C with primary antibodies against GAPDH (#2118), RUNX2 (#8486), AKT (#9272), p-AKT (Ser 473; #4060), FOXO1 (#2880), and phospho-FOXO1 (Ser256; #9461) (all from Cell Signaling Technology

(7) GIPC2 regulation of the PKM2/SREBP1 signaling axis controls adipogenic differentiation of mesenchymal stem cellsCell Death & DiseaseJanuary 7, 2026Jiayi Wang, Chengqi Xin, Zhaokai Sun, Mengke Zhao, Yaoyao Zan, Zhongyue Lv, Shuaiyu Zhu, Jing Liu, Liang Wang1:1000), SOX-9 (Abcam, ab185966, 1:5000), Collagen II (Proteintech, 28459-1-AP, 1:1000), RUNX 2 (CST, 8486, 1:1000), IBSP (Invitrogen, PA5-114915, 1:1000), Phospho-PKM2 (Invitrogen, PA5-37684, 1:1000), Histone H3

(8) Pharmacological Enhancement of Integrated Stress Response Confers Protection in Calcific Aortic Valve DiseaseJACC Basic Transl SciDecember 15, 2025Libo Wang, Xulei Duan, Huibing Liu, Fei Lin, Chaoyuan Zhou, Katrin Schröder, Ajay M. Shah, Guoan Zhao, Min ZhangWestern blot analysis as described previously.27 The primary antibodies used were: Nox4 (ab109225, Abcam); Runx2 (8486, Cell Signaling); Osteopontin (ab8448, Abcam); p-eIF2α (ab32157, Abcam); eIF2α (11170-1-AP, Proteintech);

(9) Mechanosensor Piezo1‑mediated smooth muscular cell pyroptosis contributes to vascular calcificationInternational journal of molecular medicineNovember 1, 2025Tao J, You D, Feng Z, Li H, Zhang Y, Cui Y, Lin K, Luo B, Yin S, Tan H.LLC; Bio-Techne; cat. no. NBP1-19751; 1:2,000), anti-RUNX2 (Cell Signaling Technology, Inc.; cat. no. 8486s; 1:2,000), anti-β-actin (Cell Signaling Technology, Inc.; cat. no. 3700S; 1:5,000), anti-NLRP3 (Adipogen

(10) Co-treatments of vitamin D and mesenchymal stem cells effectively alleviate the diabetic kidney disease through attenuating the SIRT1-mediated pathwaysStem Cell Res TherAugust 26, 2025Xinfeng Zhao, Sirui He, Xin Luo, Xiaru Zhang, Xueyi Jiang, Yueqing Liang, Tiantian Tang, Kemin Qi, Yang Wang, Yi Zhang, et al.C/EBPα (no. 2295, Cell Signaling Technology, 1:1000), ALP (no. sc-365765, Santa Cruz, 1:100), Runx2 (no. 8486, Cell Signaling Technology, 1:1000), VDR (no. 12550, Cell Signaling Technology, 1:1000), CYP27B1 (no.

(11) miR-424/322 attenuates cardiac remodeling by modulating the nuclear factor-activated T-cell 3/furin pathwayBiomedical journalAugust 1, 2025Tsai HY, Wang JC, Hsu YJ, Lin CY, Huang PH, Tsai MC, Hsu CW, Yang SF, Tsai SH.collected using FA lysis buffer. Sheared chromatin was used for immunoprecipitation with NFATc3 (CST, #8486) or immunoglobulin G (IgG, CST, #2729) antibody followed by purification using Protein A/G Dynabeads

(12) Inhibition of CD45-specific phosphatase activity restores the differentiation potential of aged mesenchymal stromal cells: implications in regenerative medicineBiol ResMay 2, 2025Madhurima Das, Isha Behere, Ganesh Ingavle, Anuradha Vaidya, Vaijayanti Prakash Kalelaboratories, India) in 1× PBS for 1 h at room temperature and then incubated with rabbit anti-RUNX2 (Cat no. 8486), -PPARG (Cat no. 2435) and -NF-κβ (Cat no. 8242) antibodies (Cell Signaling Technologies, MA, USA)

(13) Mechanical sensing protein PIEZO1 controls osteoarthritis via glycolysis mediated mesenchymal stem cells-Th17 cells crosstalkCell Death DisApril 1, 2025Yikun Zhou, Mingzhao Li, Shuai Lin, Zilu Zhu, Zimeng Zhuang, Shengjie Cui, Liujing Chen, Ran Zhang, Xuedong Wang, Bo Shen, et al.IFN-γ(Invitrogen, PA5-119649), MIF (Biolegend, 524001), ALP (Abcam, ab15411), RUNX2 (Cell Signaling Technology, 8486), SOX9 (Abcam, ab27414), ACAN (Sigma, MABT83) and β-actin (ZSGB-bio, TA09). The TMJOA animal model Rats

(14) The Calcimimetic R568 Reduces Vascular Smooth Muscle Cell Calcification in Vitro Via ERK 1/2 PhosphorylationInt J NephrolMarch 18, 2025Jonas Engeßer, Philipp Gregor Albert, Matthias Scheuch, Norina Loth, Sylvia Strackeserum albumin in TBS-T for 1 h at room temperature. Incubation with primary antibody against RUNX2 (#8486 Cell Signaling Technology, Europe), GAPDH (GE Healthcare UK), p44/42 MAPK (ERK 1/2) antibody (#9102

(15) The effect of osteoclasts on epigenetic regulation by long non-coding RNAs in osteoblasts grown on titanium with nanotopographyBiomaterials AdvancesMarch 1, 2025Rayana Longo Bighetti-Trevisan, Luciana Oliveira Almeida, Jaqueline Isadora Reis Ramos, Gileade Pereira Freitas, Fabiola Singaretti Oliveira, Jonathan Alexander Robert Gordon, Coralee Elizabeth Tye, Gary Stephen Stein, Jane Barbara Lian, Janet Lee Stein, et al.PA5-86886; Thermo Fisher Scientific, Waltham, MA, USA), anti-RUNX2 (1:2000; rabbit monoclonal antibody, 8486; Cell Signaling, Danvers, MA, USA), anti-ALPL (1:3000; rabbit monoclonal antibody, ab108337; Abcam,

(16) Gingival fibroblast suppress the osteogenesis process mediated by bone substitute materials via WNT/β-catenin signaling pathway in vitro and in vivoFront Bioeng BiotechnolFebruary 10, 2025Guanqi Liu, Jiahui Lin, Xiaoyan Chen, Runheng LiuAbcam, Cambridge, United Kingdom), OPN (#ab166709, 1:1,000, Abcam, Cambridge, United Kingdom), RUNX2 (#8486, 1:1,000, CST, Danvers, Massachusetts, United States), VEGF (#ab46154, 1:1,000, Abcam, Cambridge, United

(17) The inhibition of PINK1/Drp1-mediated mitophagy by hyperglycemia leads to impaired osteoblastogenesis in diabetesiScienceJanuary 17, 2025Xiao-jing Chen, Yu-ying Yang, Zheng-can Pan, Jing-zun Xu, Tao Jiang, Lin-lin Zhang, Ke-cheng Zhu, Deng Zhang, Jia-xi Song, Chun-xiang Sheng, et al.antibody,Novus,Cat# BC100-494S Rabbit Anti-Drp1 antibody,Abcam,Cat# ab184247 Rabbit Anti-Runx2 antibody,CST,Cat# 8486 Rabbit Anti-Osx antibody,Abcam,Cat# ab209484 Anti-VDAC1/Porin antibody,Abcam,Cat# ab306581 LC3A/B antibody

(18) The microbial metabolite imidazole propionate dysregulates bone homeostasis by inhibiting AMP-activated protein kinase (AMPK) signalingCommunications BiologyDecember 19, 2024Suk-Gyun Park, Jung-Woo Kim, Ju Han Song, Seung-Hee Kwon, Sin-Hye Oh, Xianyu Piao, Zhao Wang, Je-Hwang Ryu, Nacksung Kim, Ok-Su Kim, et al.(#2430 s), anti-rabbit adiponectin (#2789 s), anti-rabbit FABP4 (#2120 s), and anti-rabbit RUNX2 (#8486 s) were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-mouse OSX (sc-393325), and

(19) Identification and validation of the diagnostic biomarker MFAP5 for CAVD with type 2 diabetes by bioinformatics analysisFrontiers in ImmunologyDecember 19, 2024Qiang Shen, Lin Fan, Chen Jiang, Dingyi Yao, Xingyu Qian, Fuqiang Tong, Zhengfeng Fan, Zongtao Liu, Nianguo Dong, Chao Zhang, et al.reagents The following antibodies were used for western blotting and immunofluorescence: RUNX2 (CST, 8486), ALPL (R&D, MAB1448), GAPDH (Proteintech, 60004-1-Ig), MFAP5 (Proteintech, 15727-1-AP), goat anti-rabbit

(20) Long Noncoding RNA EMX2‐AS Facilitates Osteoblast Differentiation and Bone Formation by Inhibiting EMX2 Protein Translation and Activating Wnt/β‐Catenin PathwayStem Cells InternationalNovember 26, 2024Linyuan Fan, Li Zhang, Xin Zhang, Wei Wei, Zhaohui Liu#ab108337), OPN polyclonal antibody (Abcam, #ab69498), RUNX2 polyclonal antibody (Cell Signaling Technology, #8486), IBSP polyclonal antibody (Cell Signaling Technology, #5468), BMPR2 polyclonal antibody (Cell Signaling Technology

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RUNX2 (D1H7) Rabbit mAb

RUNX2 (D1H7) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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RUNX2 (D1H7) Rabbit mAb from Cell Signaling Technology

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