Fig 1: Effects of RSV on senescence induced by irradiation. (A-C) Histological assessment of the liver senescence by β-gal staining. (A) β-gal staining for detecting the senescent cells on day 180 (upper panel) and day 30 (lower panel) after the irradiation. (B) The apparent senescence-positive cells in the RT+vehicle group on day 30 and day 180 after irradiation (arrow). (C) Quantification of the senescent cells on day 30 and day 180 after irradiation. Error bars depict SEM. * p < 0.05, ** p < 0.01 by one-way ANOVA (n = 6, repeat 3times). Representative β-gal-stained liver images from five experimental groups (nuclear staining by new fast red). Scale bar: 50 μm. (D) Western blot for P16 and P21 in mice livers on day 30 and day 180 after the irradiation. Actin was used as an endogenous control.