Fig 1: RTECs express and release CCN1 following renal I/R injury.A A diagram illustrating the setup of the experiment uxIRI. B Representative images of H&E staining, KIM1, F4/80, and CCN1 immunostaining in kidney tissues. C Renal CCN1 protein and mRNA expression. D Serum CCN1 levels in mice after I/R. n = 4 mice per group. E CCN1 expression in RTECs from AKI patients by single-cell RNA-seq. F Representative fluorescent micrographs and quantification analysis of CCN1 (yellow), AQP1 (red), and F4/80 (green) staining in I/R-AKI mice kidney. n = 4 mice per group. G CCN1 mRNA expression levels in HK-2 cells after H/R or H2O2 stimulation. H CCN1 protein expression levels in HK-2 cells after H/R or H2O2 stimulation. I Secreted CCN1 protein levels in culture supernatants of HK-2 cells after H/R or H2O2 stimulation. n = 3 per group. Data are presented as mean ± SD.