Western blot of Rabbit Anti-Adenosine Deaminase (Calf Spleen) antibody. Lane 1: Adenosine Deaminase. Load: 50ng. Primary antibody: Adenosine Deaminase antibody at 1:1,000 for overnight at 4ÃäC. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 44 kDa for Adenosine Deaminase. Other band(s): None.