Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1) and HeLa (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 2 µg/mL) and detected by chemiluminescence of alkaline phosphatase (AP) using Rabbit anti-Mouse IgG (H+L) Secondary Antibody, AP conjugate (Product # 31329) at dilutions 1:5,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection of alkaline phosphatase (AP) was performed using Novex® AP Chemiluminescent Substrate (CDP-Star®) (Product # WP20002) with Novex® AP Chemiluminescent Substrate Enhancer (Nitro Block II™) (Product # WP20003).