Western Blot of Rabbit Anti-Ffar4 antibody. Lane 1: Ladder Opal Prestained (p/n MB-210-0500). Lane 2: Raw 264.7 WCL (p/n W10-001-369). Lane 3: NIH/3T3 WCL (p/n W10-000-358). Lane 4: PC-12 WCL (p/n W12-001-GL9). Lane 5: Ladder Opal Prestained (p/n MB-210-0500). Load: 35 µg per lane. Primary antibody: Ffar4 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Gt-a-Rb HRP secondary antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Block: (p/n MB-073) BlockOut Universal Blocking Buffer for 30 min at RT. Predicted/Observed size: 40.8 kDa, observed ~48 kDa band due to glycosylation of Ffar4 protein.