Fig 1: Levels of NF186 and related proteins in the Mgat5b KO mouse brain.a Western blotting of NF186 in brain homogenates from 10- to 30-week-old WT and Mgat5b KO mice. Samples were probed with antibodies against NF186 and GAPDH. Signal intensity for NF186 was quantified and normalized against that of GAPDH, and the values relative to WT are shown (n = 6 mice; mean ± S.D.; n.s. not significant; unpaired t-test). b Real-time PCR analysis of the Nfasc mRNA levels in the brains of 10-week-old WT and Mgat5b KO mice. The expression levels were normalized against those of 18S rRNA, and the values relative to WT are shown (n = 5 mice; mean ± S.D.; n.s. not significant; unpaired t-test). c Western blotting of myelin-related proteins in brain homogenates from 10- to 30-week-old WT and Mgat5b KO mice. Samples were probed with antibodies against Caspr, Contactin 1, and MAG. Signal intensities were quantified, and the values relative to WT are shown (n = 6 mice; mean ± S.D.; n.s. not significant; unpaired t-test). d Immunofluorescence staining of brain white matter (region (iv) in Fig. 1c) in mouse brain sections with antibodies against Caspr (red) and NF186 (green). Scale bar: 5 μm. Created in BioRender. Kizuka, Y. (2026) https://BioRender.com/toa6hhs. e Schematic illustration of the region measured as the width of the NF186-positive region. Violin plots quantifying the width of the NF186-positive region within the white matter. Measurements were taken from brain sections stained as described in (a). (n = 200 nodes in 10 WT or 6 KO mice, ***p < 0.001; unpaired t-test).