Fig 2: Effect of RCC2 expression on MCF-7 cells. MCF-7 cells were cultured and transfected with anti-RCC2 siRNA (si-RCC2) or RCC2-expressing plasmids (O-RCC2). AllStars siRNA (si-AllStars) and RFP-expressing plasmids (O-RFP) were used as the respective controls. (a, b) Cell proliferation was measured using the CCK-8 assay. (c, d) Apoptosis was measured using flow cytometry. (e, f) Cell migration ability was measured using a wound healing assay. ∗p < 0.05 and ∗∗p < 0.01.

Fig 3: RCC2 expression levels are upregulated in the urogenital male reproductive system and were associated with poor clinical prognosis. (A) Scatter plot of RCC2 in prostate cancer and normal tissues. (B) Scatter plot of RCC2 in bladder urothelial carcinoma and normal tissues. (C) Scatter plot of RCC2 in renal clear cell carcinoma and normal tissues. RCC2 in (D) patients with prostate cancer, (E) patients with bladder urothelial carcinoma and (F) patients with kidney clear cell carcinoma data was analyzed by Kaplan-Meier analysis (***P<0.001). RCC2, regulator of chromosome condensation 2; N, normal; T, tumor.

Fig 4: High expression levels of RCC2 in endometrial cancer and sarcoma are correlated with worse clinical outcomes. Scatter plot of RCC2 in (A) endometrial cancer and normal tissues and (B) sarcoma and normal tissues. Kaplan-Meier analysis of RCC2 in patients with (C) endometrial cancer and (D) sarcoma (*P<0.05, ***P<0.001). RCC2, regulator of chromosome condensation 2; N, normal; T, tumor.

Fig 5: Expression of RCC2 in breast tumor tissues. (a) Western blot analysis detected an immunosignal with a molecular weight of 56 kDa in ER + breast tumor tissues (n = 13) and breast fibroadenoma (n = 7). The analysis also detected an immunosignal at a molecular weight of 37 kDa using anti-GAPDH in these tissue samples. (b) RCC2 expression was normalized with GAPDH expression. (c) Immunohistochemistry detected significant RCC2 expression in breast tumor tissues. The analysis also detected the expression of RCC2 in endothelial cells of normal breast tissues. Original magnification: 200x. (d) Semiquantification of RCC2 expression in breast tumor and healthy breast tissues. The y-axis indicates the level of RCC2 expression. ∗∗∗p < 0.001.