Fig 2: Loss of DJ-1 increases the expression of cytoskeletal protein Palladin. a Schematic depiction of the experimental approach. b Volcano plots of proteomic data. Results for decidualized EnSC with or without siDJ-1 transfection are shown (n = 4). Orange circles show proteins which have significant increases. Green circles show proteins which have significant decreases. Black circles are proteins without any differences. Significantly regulated proteins were defined by a log₂ fold change > 0.11 or < –0.11 and raw p‑value < 0.05. c Expression of DJ-1 and PALLD in decidualized EnSC with or without siDJ-1 transfection, presented as LFQ intensity. Box represents the interquartile range (IQR), line indicates the median, whiskers show 1.5 × IQR, unpaired t-test was used to calculate statistical significance. ***P < 0.001. d Western blot analysis of Palladin expression in decidualizing EnSC with or without transfection of siDJ-1 (n = 6). GAPDH was used as a loading control. The data are presented as mean ± SEM. One-way ANOVA was used to calculate statistical significance. *P < 0.05. e GO enrichment analysis of differentially expressed proteins. GO molecular function enrichment analyses of up-regulated (orange) and down-regulated (green) proteins were performed. f KEGG enrichment analysis up-regulated (orange) and down-regulated (green) proteins were performed