ATF-3 (D2Y5W) Rabbit mAb 33593T from Cell Signaling Technology

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Cell death & disease
October 13 2023

Fig 1: Lowering ATF3 improves the effect of 5-FU on CRC MCTS.A Plot for protein interactions among genes ATF3, ATF4, DDIT4, TRIB3 and FGD6 by the STRING interaction network. B Expression levels of ATF3, ATF4, DDIT4 and TRIB3 in HCT116, DLD-1 and HT-29 cells treated with DMSO or 100 nM sangivamycin for 24 h under monolayer conditions. Actin is the loading control. C Expression levels of ATF3, DDIT4 and TRIB3 were assessed in COAD tissues and normal adjacent tissues using RNA-Seq data from TCGA (* p < 0.05). Cohort size: (num)normal adjacent tissues = 349; (num) COAD tissues = 275. D Western blot analysis of the co-IP complex from cells treated with DMSO or 100 nM sangivamycin for 24 h with ATF3 antibody immobilized on Dynabeads®. Tubulin is the loading control for the input conditions. E Expression levels of ATF3, ATF4, DDIT4 and TRIB3 in HCT116 ATF3parental and ATF3KO cells treated with DMSO or 100 nM sangivamycin for 24 h under monolayer conditions. Actin is the loading control. F Illustration of the interaction between ATF3, ATF4, DDIT4 and TRIB3 based on our results and findings from other groups [79–81]. G ATF3 expression levels in HCT116 and HT-29 cells treated with the indicated concentrations of rapamycin for 72 h under monolayer conditions. Actin is the loading control. H ATF3 expression levels in HCT116 MCTS cells treated with rapamycin (10 µM) or Torin-1 (0.5 µM) for 72 h. Actin is the loading control. I Dose response of HCT116 and HT29 MCTS to 5-FU. The data is expressed as the mean ± SD (n = 3). The half-maximal inhibitory concentration (IC50) was calculated using nonlinear regression, [Inhibitor] vs. normalized response analysis. J Combination of rapamycin (10 µM) or Troin1(0.5 µM) with 5-FU under HCT116 MCTS conditions for 72 h. The data is expressed as the mean ± SD (n = 3, unpaired t-test, two-stage step-up (Benjamini, Krieger, and Yekutieli); * p < 0.05, *** p < 0.001). K Combination of rapamycin (10 µM) or Troin1(0.5 µM) with 5-FU under HT29 MCTS conditions for 72 h. CDI = AB/(A × B) where: AB = cell viability value for the combination of rapamycin/torin-1 and 5-FU. A and B = cell viability value for the single treatment rapamycin/torin-1 or 5-FU. A CDI value <1, =1 or >1 indicates that the drugs are synergistic, additive, or antagonistic, respectively. A CDI value less than 0.7 indicates a significant synergetic effect [31].
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Cancer science
May 01 2020

Fig 2: Upregulation of activating transcription factor 3 (ATF3) by Epstein‐Barr virus (EBV) factors. A, Relative expression of ATF3 in conditions of overexpression of EBV factors. Each EBV factor, known to express in latent infection in EBV+ gastric cancer, was overexpressed in MKN7 cells and expression level of ATF3 was analyzed by RNA sequencing (RNA‐seq). ATF3 was most upregulated by overexpression of EBV‐determined nuclear antigen 1 (EBNA1), and >1.5‐fold by EBV‐encoded small RNA 1 (EBER1) and latent membrane protein 2A (LMP2A). B, EBNA1 target region for ATF3. Previous ChIP‐seq data for EBNA1 binding regions showed that EBNA1 could bind to an enhancer region upstream of ATF3. C, EBNA1 inhibitor. Overexpression of mutant EBNA1, known as EBNA1 inhibitor, in MKN7_EB cells resulted in decrease of the copy number of EBV genome and ATF3 expression
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Experimental & molecular medicine
November 01 2021

Fig 3: Increased HOXA11-AS expression suppressed ATF3 transcription in GC cells.a Heatmap of altered lncRNAs (fold change > 4, p < 0.05) in ATF3low GC tissues compared with paired adjacent normal tissues. Red arrows indicate a significant increase in lncRNA HOXA11-AS expression in ATF3low GC tissues. b The expression level of HOXA11-AS in GC tissues compared with that in paired normal gastric tissues as detected by RT-qPCR in our GC samples. c Correlation between the mRNA levels of HOXA11-AS and ATF3 in the GEO GSE35809 dataset (i) and in our GC samples (ii). d The expression level of HOXA11-AS was detected by RT-qPCR in HOXA11-AS-knockdown MGC-803 and MKN-45 cells. e ATF3 mRNA (i) and protein levels (ii) were detected by RT-qPCR and western blot, respectively, in HOXA11-AS-knockdown MGC-803 and MKN-45 cells, with β-actin serving as an endogenous control. f RT-qPCR and western blotting were performed to detect the mRNA (i) and protein (ii) levels, respectively, of β-catenin in HOXA11-AS-knockdown MGC-803 and MKN-45 cells, with β-actin serving as an endogenous control. g RIP analyses of HOXA11-AS1 and its predicted binding partners in MGC-803 (i) and MKN-45 cells (ii). h The colocalization of HOXA11-AS and TCF3 was analyzed by concomitant FISH and IF in MGC-803 and MKN-45 cells. Red, HOXA11-AS; Green, TCF3; blue, DAPI. Scale bar: 10 μm. i Western blotting analyses of TCF3 expression in MKN-45 cells subjected to pull-down with full-length HOXA11-AS. j ChIP-qPCR analysis of TCF3, EZH2, and H3K27me3 occupancy in the ATF3 promoter in MGC-803 and MKN-45 cells. k ChIP-qPCR analysis of TCF3, EZH2, and H3K27me3 occupancy in the ATF3 promoter in HOXA11-AS-knockdown MGC-803 cells. *p < 0.05; **p < 0.01 and ***p < 0.001 by Student’s t test compared with the corresponding control group.
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Clinical and translational medicine
April 01 2024

Fig 4: Serum activating transcription factor 3 (ATF3) is elevated in clinical spinal cord injury (SCI) and ischaemic stroke patients 24 h after injury. (a) Human serum ATF3 levels were measured using a commercially available enzyme‐linked immune‐sorbent assay (ELISA) kit in healthy control (n = 7), trauma control patients without SCI or traumatic brain injury (TBI) 24 h after injury (n = 7), and SCI patients 24 h after injury (n = 30). Serum ATF3 levels in SCI patients were significantly higher than those in healthy control and trauma control patients, with no statistical difference between healthy control and trauma control groups. (b) The serum ATF3 levels in patients with different severity of SCI. American Spinal Injury Association Impairment Scale (AIS) D represents mild SCI, while AIS A indicates the most severe SCI. (c) Human serum ATF3 levels were measured by ELISA from non‐stroke patient controls (n = 8) and patients within 24 h of ischaemic stroke (n = 21). Serum ATF3 levels were significantly elevated in stroke patients. (d) The serum ATF3 levels in patients with different NIH Stroke Score/Scale (NIHSS) at 24 h. Data are presented as mean ± SEM and are analysed with one‐way analysis of variance (ANOVA) with Bonferroni's multiple comparison tests (a, b and d) or unpaired two‐tailed t‐test (c), **** p < .0001, *** p < .001, ** p < .01, * p < .05 and ‘ns’ as not statistically significant.
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Cell death & disease
May 06 2024

Fig 5: Mechanism diagram.ATF3 promotes transcription of ABCB4 in GSCs, leading to ABCB4 overexpression, thereby promoting resistance of GSCs to TMZ. Concurrently, GSCs transfer ABCB4 to DGCs via exosomes, so that DGCs also increase resistance to TMZ. MVB multivesicular body.

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Cell Signaling Technology for
ATF-3 (D2Y5W) Rabbit mAb

The ATF-3 (D2Y5W) Rabbit mAb Antibody from Cell Signaling Technology is a Rabbit Monoclonal antibody to ATF3. This antibody recognizes Homo Sapiens, Human, and Mouse antigen. The ATF-3 (D2Y5W) Rabbit mAb Antibody has been shown to work in the following applications: Precipitation, ChIP, Immunoprecipitation, and Western Blot.

Product Specs
ItemATF-3 (D2Y5W) Rabbit mAb
CompanyCell Signaling Technology
Price $153.00 Inquire
Catalog Number33593T
ClonalityMonoclonal
ReactivityH, M
ApplicationsW, ChIP
HostRabbit
Quantity20 µl
TargetATF-3
NCBI Full Gene Nameactivating transcription factor 3

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(1) The lysosomal LAMTOR-Rag complex functions as a checkpoint for antiviral interferon productionThe EMBO JournalJanuary 22, 2026Zeming Feng, Lulu Wang, Shujun Chen, Sihan Cao, Miao Lei, Xiuzhen Yang, Kaixiong Ma, Shi Yu, Huina Hu, Kaixuan Zheng, et al.(IB 1:1000),Cell Signaling Technology,#72604 (D9J7L) ATF-3 (IB 1:1000),Cell Signaling Technology,#33593 (D2Y5W) VDAC (IB 1:1000),Cell Signaling Technology,#4661 (D73D12) Golgin-97 (IB 1:1000),Cell Signaling Technology

(2) Acinar-specific loss of activating transcription factor 3 restricts KRASG12D mediated transcriptional changes and PanIN progressionCell Death DiscoveryNovember 6, 2025Mickenzie B. Martin, Fatemeh Mousavi, Gavin Goebel, Domenic Di Stasi, Alesia Mano, Andrei Glogov, Liena Zhao, Gianni Di Guglielmo, Parisa Shooshtari, Christopher L. PinPrimary antibodies for tERK (1:500, Cell Signaling, cat. #9102S), ATF3 (1:1000; Cell Signaling, cat #33593S), Bcl-xL (1:1000; Abcam cat #ab32370), cleaved caspase 3 (1:500; Cell Signaling, cat #9661S), E-cadherin

(3) PACT suppresses PKR activation through dsRNA binding and dimerization, and is a therapeutic target for triple-negative breast cancerRNANovember 1, 2025Addison A. Young, Isabelle G. Juhler, Jackson R. Pierce, Holly E. Bohlin, Haley A. Harper, David S. Onishile, Renee N. Chua, Madison E. Liu, Estelle N. Gardner, Bennett D. Elzey, et al.sc-73408, RRID:AB_2222767; Bethyl Cat# A303-883A, RRID:AB_2620233), ATF3 (Cell Signaling Technology Cat# 33593, RRID:AB_2799039), eIF2a (Abcam Cat# ab5369, RRID:AB_304838), eIF2a-Ser-51-P (Abcam Cat# ab32157, RRID:AB_732117),

(4) Programmed cell revival from imminent cell death enhances tissue repair and regenerationThe EMBO JournalAugust 21, 2025Kollori Dhar, Kautilya Kumar Jena, Subhash Mehto, Rinku Sahu, Krushna C Murmu, Atharva Anand Mahajan, Sibaram Behera, Ravi Kiran Putchala, Reuben Jacob Mathew, Ramyasingh Bal, et al.(Rabbit-pAb),GeneTex,Cat# GTX134238; RRID:AB_2887243 ATF3 (D2Y5W) (Rabbit-mAb),Cell Signaling Technology,Cat#33593; RRID:AB_2799039 Histone H3 (Rabbit-pAb),Invitrogen,Cat# PA5-17697; RRID:AB_10980629 Anti-LC3B (Rabbit-pAb),Millipore-Sigma,Cat#

(5) Integrated bioinformatics and experimental validation identify ATF3 as a key gene in secondary brain damage after intracerebral hemorrhagePLoS OneJuly 18, 2025Tao Cui, Jinbang Huang, Chaoyong Zhang, Bin Wangcells (80 μM heme, 24 hr) showing nuclear ATF3 accumulation (red; rabbit anti-ATF3, Cell Signaling 33593, 1:200) vs. vehicle control (0.1% DMSO). Nuclei counterstained with Hoechst 33342 (blue). Scale bars:

(6) ONC201 enhances the cytotoxic effect of cisplatin through ATF3/ATF4/CHOP in head and neck squamous cell carcinoma cellsOncogenesisJune 18, 2025Hui-Ching Chuang, Ming-Hsien Tsai, Jiin-Haur Chuang, Ya-Ting Hong, Chih-Yen Chien, Ming-Huei Chouwere then incubated overnight at 4°C with primary antibodies against ATF3 (Cell Signaling Technology, #33593), γ-H2AX (Abcam, ab11174), ATF4 (Cell Signaling Technology, #11815), CHOP (Cell Signaling Technology,

(7) Integrator loss leads to dsRNA formation that triggers the integrated stress responseCellJune 12, 2025Apoorva Baluapuri, Nicole ChenCheng Zhao, Ryan J. Marina, Kai-Lieh Huang, Anastasia Kuzkina, Maria E. Amodeo, Chad B. Stein, Lucie Y. Ahn, Jordan S. Farr, Ashleigh E. Schaffer, et al.RRID: AB_2263076, Lot# 00107128" Rabbit monoclonal anti-ATF-3 (D2Y5W),Cell Signaling Technology,"Cat# 33593, RRID: AB2799039, Lot# 2" Rabbit monoclonal anti-ATF-4 (D4B8),Cell Signaling Technology,"Cat# 11815,

(8) The PERK-ATF4 pathway is required for metabolic reprogramming and progressive lung fibrosisJCI InsightMay 22, 2025Jyotsana Pandey, Jennifer L. Larson-Casey, Mallikarjun H. Patil, Chao He, Nisarat Pinthong, A. Brent Carterfor input controls (2% of total) or for immunoprecipitation of ATF3 (D2Y5W) anti-rabbit monoclonal (33593; Cell Signaling Technology), ATF4 anti-rabbit polyclonal (10835-1-AP; Proteintech), histone H3 (D1H2)

(9) Bisphenol S Exposure and MASLD: A Mechanistic Study in MiceEnvironmental health perspectivesMay 1, 2025Li S, Fan Y, Tang M, Wu X, Bai S, Yang X, Zhang X, Lu C, Ji C, Wade PA, et al.blocked with 5% skim milk at room temperature for 1 h and incubated at 4°C overnight with the Atf3 (CST; 33593), Gapdh (CST; 5174), and Actb (Affinity; AF7018) at a dilution of 1:1,000. After washing with PBST,

(10) Activating transcription factor 3 regulates hepatic apolipoprotein A4 upon metabolic stressJournal of Biological ChemistryMay 1, 2025Jasmine Encarnacion, Danielle M. Smith, Joseph Choi, Joseph Scafidi, Michael J. Wolfgangprimary antibodies in 3% bovine serum albumin overnight at 4 °C; ApoA4 (#AF8125, R&D systems), Atf3 (#33593, Cell Signaling), and HSC70(sc-7298, Santa Cruz). Blots were incubated with secondary antibodies in

(11) Exosomes secreted by ATF3/Nrf2-mediated ferroptotic renal tubular epithelial cells promote M1/M2 ratio imbalance inducing renal interstitial fibrosis following ischemia and reperfusion injuryFront ImmunolFebruary 5, 2025Qiao Tang, Jiatao Xie, Yifei Wang, Chong Dong, Qian Sunincubated with β-actin antibody (1: 1000, Servicebio, GB11001-100, China), ATF3 antibody (1: 1000, CST, 33593S, USA), Nrf2 antibody (1: 1000, protein, 16396-1-AP, USA). After washing with TBST for 30 minutes, PVDF

(12) Endothelial activating transcription factor 3 promotes angiogenesis and vascular repair in the mouse retinaiScienceJanuary 17, 2025Chihiro Ueda, Susumu Sakimoto, Masahito Yoshihara, Toru Takigawa, Akihiko Shiraki, Kaito Yamaguchi, Kosuke Shiki, Nobuhiko Shiraki, Shigetaka Kitajima, Yoshiaki Kubota, et al.anti-ki67,Invitrogen,Cat#: MA5-14520; RRID: AB_10979488 Rabbit anti-ATF3,Cell Signaling Technology,Cat# 33593; RRID: AB_2799039 Mouse anti-βactin,Sigma-Aldrich,Cat#: A5441; RRID: AB_476744 Alexa Flour 488 goat anti-rat IgG

(13) Pharmacological Modulation of the Unfolded Protein Response as a Therapeutic Approach in Cutaneous T-Cell LymphomaBiomoleculesJanuary 7, 2025St Thomas N, Christopher BN, Reyes L, Robinson RM, Golick L, Zhu X, Chapman E, Dolloff NG.Primary Antibodies,, Protein Target,Vendor,Catalog # β-actin,Sigma,A5441 ATF3,Cell Signaling,33593S ATF4,Cell Signaling,11815S BiP,Cell Signaling,3183S CHOP,Cell Signaling,2895S eIF2α,Cell Signaling,5324T p-eIF2α,Cell Signaling

(14) Integrated stress response plasticity governs normal cell adaptation to chronic stress via the PP2A-TFE3-ATF4 pathwayCell Death & DifferentiationDecember 1, 2024Rita A. Avelar, Riya Gupta, Grace Carvette, Felipe da Veiga Leprevost, Medhasri Jasti, Jose Colina, Jessica Teitel, Alexey I. Nesvizhskii, Caitlin M. O’Connor, Maria Hatzoglou, et al.Cell Signaling: BiP (3177), PERK (3192), p-eIF2α Ser51 (3597), t-eIF2α (2103), ATF4 (11815), ATF3 (33593), TRIB3 (43043), DR5 (8074), cleaved Caspase 3 (9661), cleaved PARP (9541), LC3I/II (12741), PP2A-Aα

(15) Glioblastoma stem cells deliver ABCB4 transcribed by ATF3 via exosomes conferring glioblastoma resistance to temozolomideCell death & diseaseMay 6, 2024Xu X, Zheng Y, Luo L, You Z, Chen H, Wang J, Zhang F, Liu Y, Ke Y.to 1000 base pairs. Protein–DNA complexes were precipitated using an Anti-ATF3 antibody (CST, USA, #33593) and control IgG. Procedures were performed in accordance with the Pierce Agarose ChIP Kit guidelines

(16) ATF3 is a neuron-specific biomarker for spinal cord injury and ischaemic strokeClinical and translational medicineApril 1, 2024Pan JZ, Wang Z, Sun W, Pan P, Li W, Sun Y, Chen S, Lin A, Tan W, He L, et al.nuclei, 1:500, Millipore, #MAB377) and mouse ATF3 rabbit monoclonal antibody (1:200, Cell Signalling, #33593) at 4°C overnight. Subsequently, they were incubated with secondary antibodies, either Alexa Fluor 594‐conjugated

(17) OGDH and Bcl-xL loss causes synthetic lethality in glioblastomaJCI InsightMarch 14, 2024Trang T.T. Nguyen, Consuelo Torrini, Enyuan Shang, Chang Shu, Jeong-Yeon Mun, Qiuqiang Gao, Nelson Humala, Hasan O. Akman, Guoan Zhang, Mike-Andrew Westhoff, et al.1:500), β-actin (Sigma-Aldrich, catalog A1978, clone AC15; 1:3,000), ATF3 (clone D2Y5W) (CST, catalog 33593; 1:500), ATF4 (clone D4B8) (CST, catalog 11815; 1:500), PARP (clone 46D11) (CST, catalog 9532; 1:500),

(18) Identification of ATF3 as a novel protective signature of quiescent colorectal tumor cellsCell death & diseaseOctober 13, 2023Lu X, Zhong L, Lindell E, Veanes M, Guo J, Zhao M, Salehi M, Swartling FJ, Chen X, Sjöblom T, et al.MedChemExpress (NJ, USA). The primary antibodies used for western blotting were as follows: rabbit anti-ATF3 (#33593), rabbit anti-ATF4 (#118115) and rabbit anti-TRIB3 (#43043) were obtained from Cell signaling (MA, USA);

(19) MUC1-C intersects chronic inflammation with epigenetic reprogramming by regulating the set1a compass complex in cancer progressionCommunications biologyOctober 11, 2023Bhattacharya A, Fushimi A, Wang K, Yamashita N, Morimoto Y, Ishikawa S, Daimon T, Liu T, Liu S, Long MD, et al.anti-ASH2L (#ABE1972, 1:1000 dilution; Millipore), anti-NF-κB p65 (#8242S, 1:1000 dilution; CST), anti-ATF3 (33593S, 1:1000 dilution; CST), anti-c-FOS (#2250S, 1:1000 dilution; CST), anti-c-JUN (#3742S, 1:1000 dilution;

(20) KDM6A Loss Triggers an Epigenetic Switch That Disrupts Urothelial Differentiation and Drives Cell Proliferation in Bladder CancerCancer researchMarch 15, 2023Qiu H, Makarov V, Bolzenius JK, Halstead A, Parker Y, Wang A, Iyer GV, Wise H, Kim D, Thayaparan V, et al.and 1.5 μg anti-KDM6A (#33510, Cell Signaling Technology, RRID:AB_2721244) or anti-ATF3 antibody (#33593, Cell Signaling Technology, RRID:AB_2799039) were incubated with 3×105 cells overnight at 4°C. The enriched

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Cell death & disease

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Cell death & disease

ATF-3 (D2Y5W) Rabbit mAb from Cell Signaling Technology

Cell Signaling Technology

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ATF-3 (D2Y5W) Rabbit mAb from Cell Signaling Technology

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