Fig 2: Smad4 inhibits the expression of Dlg3 by directly binding to –1,049 to –776 bp in the promoter.(A) The genomic distribution of Cut&Tag peaks. (B) Smad4 gene-binding pattern. Smad4 bound with the TSS. (C) Cellular component GO enrichment analysis. (D) KEGG enrichment analysis. (E) ChIP analysis was performed to assess the interaction of Smad4 and the Dlg3 promoter. n = 3 per group. **P < 0.01 vs. IgG group. (F) Luciferase activity in HEK293T cells. n = 3 for each group. Control group (con) vs. Smad4 overexpression group (Smad4), Dlg3 promoter, ***P < 0.001; truncated Dlg3 promoter (Δ–1049~–776 bp), NS. The mRNA (G) and protein (H and I) levels of Dlg3/SAP102 in the hippocampus of AAV-sh-Smad4–treated APP/PS1 mice. n = 3. *P < 0.05 vs. AAV-con group. All data were presented as means ± SEM. Two-tailed unpaired Student’s t test (E, G, and I) and 2-way ANOVA (F) were used.