Fig 2: Histological evaluation of the wound healing. a Representative H&E staining of the wound tissues. b The granulation tissue thickness in the wound tissue (n = 3 biologically independent samples, N = 5 technical replicates). c Representative Masson staining of the wound tissue. d The collagen deposition ratio of the wound tissue (n = 3 biologically independent samples, N = 3 technical replicates). e Representative immunohistochemical staining of Collagen I in the wound tissue. f Representative immunofluorescence staining of IL-6 and TNF-α in the wound tissue. g Representative immunofluorescence staining of CD31 and α-SMA in the wound tissue, DAPI served as a marker of the cell nucleus. h Quantification of the vessel density in the wound tissue (n = 3 biologically independent samples, N = 3 technical replicates). Scale bars, 1 mm (the overall images in a, c), 200 μm (the partial images in a, c), 50 μm (images in e, f), 100 μm (images in g). ∗P < 0.05; ∗∗P < 0.01; ∗∗∗∗P < 0.0001.