Fig 1: IDH2/SRSF2 double mutants exhibit splicing differences in different genes but with a similar underlying architecture.(A) Bar plot showing the number of significant splicing differences in each genotype compared to WT samples grouped by type of event (FDR < 5% and absolute inclusion level difference > 25%). RI, retained intron; MXE, mutually exclusive exons; A5SS, alternative 5′ splice site; A3SS, alternative 3′ splice site. (B and C) Venn diagrams showing the number of CEs (B) and the number of genes with CEs (C) in each genotype. (D) Western blot analysis of selected proteins with CEs. Representative blots are shown on the left, and bar plots with the relative proteins levels are shown on the right (N = 2). The two isoforms of CCM2 are noted and were combined in the quantification. (E) Bar plot showing the percentage of exons with DMRs on or around them. Asterisks indicate statistical significance (FDR < 5%) as per hypergeometric tests. (F and G) Plots showing the cumulative distributions for CCNG (E) or GGNG (F) motifs per 1000 bp in the exons that are significantly promoted or repressed in the respective genotype with respect to WT samples as well as in all the exons included in the analysis (background). Asterisks indicate statistical significance (FDR < 5%) of the curves for promoted and repressed exons compared to the background distribution as per one-sample Kolmogorov-Smirnov tests. ns, not significant.