Fig 2: MiR-382-3p directly targeted BCAT1. (A) TargetScan (http://www.targetscan.org) predicted that miR-382-3p has binding sites with BCAT1. (B) Relative luciferase activities were estimated in U251 and HS683 cells co-transfected with BCAT1-wt or BCAT1-mut and miR-NC or miR-382-3p. ⁎⁎p <0.001, when compared to miR-NC. (C) The BCAT1 expression levels in normal human astrocytes (NHA) and U251 and HS683 cells were quantified via qRT-PCR. ⁎⁎p <0.001, when compared to NHA. (D) The levels of BCAT1 expression in the glioma and normal tissues were estimated by means of qRT-PCR. ⁎⁎p <0.001, compared to adjacent tissues. (E) The correlation among BCAT1, miR-382-3p, and PSMB8-AS1 in glioma tissues was ascertained using Pearson's correlation coefficient. (F) The levels of BCAT1 and PSMB8-AS1 in glioma cells with si-lnc or OE-BCAT1 transfection was measured using qRT-PCR. ⁎⁎p <0.001, when compared to si-NC; ##p <0.001, when compared to OE-NC; &&p <0.001, when compared to si-lnc+OE-BCAT1. All data were indicated as the mean ± SD.