Delta FosB (D3S8R) Rabbit mAb 14695T from Cell Signaling Technology

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eLife
June 21 2024

Fig 1: Choline supplementation reduced ∆FosB expression in dorsal granule cells (GCs) of Tg2576 mice.(A) Representative images of ∆FosB staining in the GCL. A section from a low choline-treated Tg2576 mouse shows robust ∆FosB-ir in the GCL. The area outlined by the box is expanded below. Red arrows point to ∆FosB-labeled cells. GCL = granule cell layer. Calibration for the top row, 100 µm; for the bottom row, 50 µm.2–3. Sections from intermediate (2) and high choline (3) -treated Tg2576 mice. Same calibrations as 1. 4, A section from a WT mouse treated with the intermediate diet. Same calibrations as 1. (B) Quantification methods. Representative images demonstrating the thresholding criteria established to quantify ∆FosB. 1. A ∆FosB-stained section shows strongly-stained cells (white arrows). 2. A strict thresholding criterion was used to make only the darkest stained cells red. (C) Use of the strict threshold to quantify ∆FosB-ir. 1. Anterior dentate gyrus (DG). Tg2576 mice treated with the choline-supplemented diet had significantly less ∆FosB-ir compared to the Tg2576 mice fed the low or intermediate diets. Tg2576 mice fed the high choline diet were not significantly different from WT mice, suggesting a rescue of ∆FosB-ir. 2. There were no significant differences in ∆FosB-ir in posterior sections. (D) Methods are shown using a threshold that was less strict. Some of the stained cells that were included are not as dark as those used for the strict threshold (white arrows). 2. All cells above the more permissive threshold are shown in red. (E) Use of the less strict threshold to quantify ∆FosB-ir. 1. Anterior DG. Tg2576 mice that were fed the high choline diet had less ΔFosB-ir pixels than the mice that were fed the other diets. There were no differences from WT mice, suggesting restoration of ∆FosB-ir by choline enrichment in early life. 2. Posterior DG. There were no significant differences between Tg2576 mice fed the three diets or WT mice.
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eLife
June 21 2024

Fig 2: Schematic of the behavioral procedures for the novel object location (NOL) and novel object recognition (NOR) tasks.(A) Experimental timeline. Dams were fed one of three diets for mating, gestation, and offspring that consumed the diet until weaning. At 1 month old, offspring were implanted with electrodes for EEG. Recordings for 24 hr started 1 week later (5 weeks old; 1.2 months old in the graphs). Additional recordings were made at 2, 3, 4, 5, and 6 months of age. At 3 and 6 months of age, behavior was tested, mice were perfused, brains were sectioned, and sections were processed with antibodies against NeuN and ∆FosB. (B) Prior to NOL or NOR, animals were acclimated to the testing arena. There were three acclimation sessions separated by 24 hr during which animals were allowed to freely explore for 5 min. (C) In the NOL task, animals were placed in a cage with two identical objects and allowed to freely explore for 5 min (Training). After 1 hr, they were brought back to the cage, where one object was displaced, and allowed to freely explore for 5 min (Testing). (D) In the NOR task. animals were placed in a cage with two identical objects and allowed to freely explore for 5 min (Training). After 1 hr, they were brought back to the cage, where one object was replaced, and allowed to freely explore for 5 min (Testing).
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Frontiers in behavioral neuroscience
January 01 2020

Fig 3: Western Blot analysis of trkB, BDNF, Δ-FosB, Arc, synaptophysin, syntaxin-3, PSD-95 and PSA-NCAM in the Acb shell and Acb core of WT, HET and DAT KO rats after the microdialysis experiment. Histograms on the left are the densitometric analyses of the marker/GAPDH band gray optical density (O.D.) ratios. Blot lines on the right are representative samples of two animals from each experimental group. Values are means ± SEM of the values obtained by the reported number of rats per group. *P < 0.05 with respect to WT rats (one-way ANOVA followed by Tukey’s pairwise comparisons).
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Frontiers in behavioral neuroscience
January 01 2020

Fig 4: Western Blot analysis of trkB, BDNF, Δ-FosB, Arc, synaptophysin, syntaxin-3, PSD-95 and PSA-NCAM in the mPFC and VTA of WT, HET and DAT KO rats after the microdialysis experiment. Histograms on the left are the densitometric analyses of the marker/GAPDH band gray optical density (O.D.) ratios. Blot lines on the right are representative samples of two animals from each experimental group. Values are means ± SEM of the values obtained by the reported number of rats per group. *P < 0.05, **P < 0.01 with respect to WT rats; #P < 0.05 DAT KO with respect to HET rats (one-way ANOVA followed by Tukey’s pairwise comparisons).
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Nature Metabolism
November 10 2025

Fig 5: Hepatic determinants associated with endogenous fructose metabolism drive ethanol intake and preference and regulate ethanol metabolism.a, Ethanol-water two-bottle choice preference ratio of male wild-type (black), Khkfl/fl×Alb1-cre (pink) and KHK-A/C KO (red) mice at three different ethanol concentrations (vol/vol 3%, 6% and 10%). b, Average ethanol intake in g kg day−1 in the same groups as in a. c, Average ethanol intake in ml per mouse per day in the same groups as in a. d,e, Cumulative 30-week ethanol intake presented over time (d) and collapsed (e). f, Schematic design of ethanol exposure (one bottle, 14 months) of ethanol pair-matched Khkfl/fl mice (7.2%, black) and Khkfl/fl×Alb1-cre (10%, pink) mice. g, Total (chow and ethanol-derived) caloric intake (one bottle, 14 months) by water control and ethanol pair-matched Khkfl/fl mice (7.2%, black) and Khkfl/fl×Alb1-cre (10%, pink) mice. h,i, Representative western blot (h) and densitometry (i) for ADH, ALDH1A1, ALDH2, AceCS1 and actin loading control in the same mouse groups as in f. j, Hepatic ALDH activity in the same mouse groups as in f. k, Time-response ethanol levels in plasma in water control (0% ethanol, left) and ethanol pair-matched (right) Khkfl/fl and Khkfl/fl×Alb1-cre mice following an oral ethanol (3 g kg−1) gavage. l, Time-response acetaldehyde levels in plasma in water control (0% ethanol, left) and ethanol pair-matched (right) Khkfl/fl and Khkfl/fl×Alb1-cre mice following an oral ethanol (3 g kg−1) gavage. m, Representative western blot for ∆FosB and GAPDH loading control in the nucleus accumbens from 14-month water control and ethanol pair-matched Khkfl/fl (7.2%) and Khkfl/fl×Alb1-cre (10%) mice. n, mRNA expression of ∆FosB target genes (Glur2 and Cdk5) in the nucleus accumbens of 14-month water control and ethanol pair-matched Khkfl/fl (black, 7.2%) and Khkfl/fl×Alb1-cre (pink, 10%) mice. The data are shown as individual values. Mean ± s.d. is additionally presented in e–l. Data were analysed in a–g, i, j and n using one-way ANOVA or two-way ANOVA (g) with Tukey’s multiple comparison post hoc test. Data in k and l were analysed using paired two-sided t-test between strains for each timepoint. Data were obtained from three separate studies and pooled together for statistical analysis. Sample size: n = 5–6 mice per group. See also Extended Data Fig. 8. Panels a and f created with Biorender.com.Source data

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Cell Signaling Technology for
Delta FosB (D3S8R) Rabbit mAb

Cell Signaling Technology's Delta FosB (D3S8R) Rabbit mAb is a Rabbit monoclonal antibody. The Delta FosB (D3S8R) Rabbit mAb Antibody was generated using FOSB as the antigen. It reacts with Homo Sapiens, Human, Mouse, Rat, and Non-Human Primate. This antibody has been shown to work in applications such as: Precipitation, Immunoprecipitation, and Western Blot.

Product Specs
ItemDelta FosB (D3S8R) Rabbit mAb
CompanyCell Signaling Technology
Price $167.00 Inquire
Catalog Number14695T
ApplicationsWB, IP
ReactivityHu, Ms, Rt, Mk
NCBI Gene AliasesAP-1, G0S3, GOS3, GOSB
NCBI Full Gene NameFosB proto-oncogene, AP-1 transcription factor subunit
Targetdelta FosB
Quantity20 µl
HostRabbit
ClonalityMonoclonal

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(1) SirT2 Inhibition is Associated with Improvements in Depression-like Behavior and Memory Impairment in Olfactory Bulbectomized MiceMol NeurobiolApril 21, 2026Kohei Takahashi, Kazuhiro Kurokawa, Daiki Hiraga, Hiroshi Takeda, Minoru Tsuji(1:1000; CST; #8782), SirT6 (1:1000; CST; #12486), SirT7 (1:1000; CST; #5360), ΔFosB (1:500; CST; #14695), HRD1 (1:500; Proteintech; 13473–1-AP), acetyl-FoxO1 (Lys294; 1:200; Invitrogen, Waltham, MA, USA;

(2) Chronic neuropathic pain alters reversal learning and prefrontal cortex activation without generalized deficits in reward-related behaviorbioRxivMarch 11, 2026Andrea Aurelio Aurelio Borges, Mitchell A. Nothem, Christina M. Curran-Alfaro, Karina Possa Abrahao, Jacqueline M. Barkertemperature with primary antibodies against c-Fos (1:5000; 2250S, Cell Signaling) or ΔFosB (1:1000; 14695 S; Cell Signaling). The next day, sections were incubated with a biotinylated donkey anti-rabbit secondary

(3) CA inhibitor 1 treatment has only transient effects on estrous cyclicity in micebioRxivJanuary 17, 2026Laura L Giacometti, Donna Mae Dalere, Bupe Lwamba, Samuel Goldberg, Kaitlyn Feliciano, Jacqueline M BarkerSigma-Aldrich, St. Louis MO, G9269) or anti-Δ fos B (1:5000, Cell Signaling Technologies, Danvers MA, 14695) overnight, followed by incubation in biotinylated donkey anti-rabbit secondary (1:500, Jackson ImmunoResearch,

(4) Alpha-2 Adrenergic Agonists Reduce Heavy Alcohol Drinking and Improve Cognitive Performance in MiceeNeuroJanuary 12, 2026Sema G. Quadir, Lauren Lepeak, Sophia Miracle, Roberto Collu, Olivia Velte, Yingchu He, Zeynep Ozturk, Christian D. Rohl, Valentina Sabino, Pietro Cottoneprimary antibody (Millipore MAB318, 1:500), and rabbit anti-DeltaFosB primary antibody (Cell Signaling 14695, 1:450) in blocking solution for 24 h at 4°C. Sections were then incubated in secondary antibodies:

(5) Identification of a common ketohexokinase-dependent link driving alcohol intake and alcohol-associated liver disease in miceNature MetabolismNovember 10, 2025Ana Andres-Hernando, David J. Orlicky, Gabriela E. Garcia, Esteban C. Loetz, Richard Montoya, Vijay Kumar, Devin P. Effinger, Masanari Kuwabara, So Young Bae, Laura Lorenzo-Rebenaque, et al.(Cell Signaling 3676; RRID:AB_2219397), ACL (Cell Signaling 4332; RRID:AB_2223744), ∆FosB (Cell Signaling; 14695; RRID:AB_2798577) and actin (Cell Signaling 4968; RRID:2313904) and visualized using an anti-rabbit

(6) Augmented orexin/hypocretin signaling underlies negative affect during acute oxycodone abstinence in ratsResearch SquareOctober 13, 2025Kimberly A. Aston-Jones, Victoria A. Stiritz, Victoria Stiritz, Akihiro Yamanaka, Hiroyuki Mizoguchi, Morgan H. Mizoguchi, Morgan Aston-Jonessections in mouse anti-orexin-A (1:500) and rabbit anti-Δ-FosB (1:1000 Cell Signaling, catalog number 14695s) in 5% NDS in PBST overnight at RT. Secondary antibodies were a cocktail of Alexa-Fluor 488-conjugated

(7) Dynamic three-dimensional culture enhances tunneling nanotubes-mediated mitochondrial transfer in mesenchymal stromal cells to accelerate wound healingJ NanobiotechnologyAugust 11, 2025Lin Ma, Xiaoxue Yang, Xiaoyao Huang, Hao Guo, Zihan Li, Siyuan Fan, Han Qin, Fanhui Meng, Peisheng Liu, Xinyu Wang, et al.anti-TGF-β (130348, Santa Cruz Biotechnology, USA, 1:200), anti-TNF-α (ab1793, Abcam, UK, 1:200) and Col1a1 (14695, Cell Signaling Technology, USA, 1:200). The secondary antibodies used were Donkey anti-Mouse IgG (H

(8) Pantothenic acid ameliorates hepatic fibrosis by targeting IGFBP6 to regulate the TGF-β/SMADs pathwayCommun BiolJuly 29, 2025Zhengxin Jin, Zhengsen Jin, Zeyu Liu, Yongcheng Yin, Yuchen Zhang, Ying Zhang, Jianning Kang, Yuepeng Fang, Wei Jiang, Bin NingIHC-related primary antibody information: α-SMA (Proteintech, 14395, 1:100 dilution); COL1A1 (Proteintech, 14695, 1:200 dilution,); p-SMAD2/3 (Cell Signaling Technology, 8828, 1:200 dilution) and IGFBP6 (Proteintech,

(9) Astrocyte Gi-GPCR signaling corrects compulsive-like grooming and anxiety-related behaviors in Sapap3 knockout miceNeuronOctober 23, 2024Soto JS, Neupane C, Kaur M, Pandey V, Wohlschlegel JA, Khakh BS.mouse anti-RFP (1:500; Rockland, 600906379); and rabbit anti-ΔFosB (1:500; Cell Signaling Technology, 14695S). Sections were then incubated with the following secondary antibodies for 2 h at room temperature (1:1,000;

(10) Choline supplementation in early life improves and low levels of choline can impair outcomes in a mouse model of Alzheimer’s diseaseeLifeJune 21, 2024Elissavet Chartampila, Karim S Elayouby, Paige Leary, John J LaFrancois, David Alcantara-Gonzalez, Swati Jain, Kasey Gerencer, Justin J Botterill, Stephen D Ginsberg, Helen E Scharfmanmonoclonal),Cat# MAB377; Millipore,RRID:AB_2313673,1:5000 Antibody,Anti-ΔFosB (rabbit monoclonal; D3S8R),"Cat# 14695, Cell Signaling",RRID:AB_2798577,1:1000 Antibody,Biotinylated horse anti-mouse IgG antibody,Cat# BP-2000;

(11) Deciphering m6A methylation in monocyte-mediated cardiac fibrosis and monocyte-hitchhiked erythrocyte microvesicle biohybrid therapyTheranosticsJune 1, 2024Jiawen Li, Li Wei, Kaifeng Hu, Yunxiang He, Guidong Gong, Qisong Liu, Yue Zhang, Kaiyu Zhou, Junling Guo, Yimin Hua, et al.F4/80 (1:2000, Abcam, Ab300421), MyD88 (1:2000, proteintech, 67969), Collagen1 (1:2000, proteintech, 14695), β-actin (1:2000, proteintech, 81115), p50 (1:2000, CST, 13586), Lamin B1 (1:2000, proteintech, 12987-1-AP),

(12) Altered Hippocampal Activation in Seizure-Prone CACNA2D2 Knock-out MiceeNeuroMay 1, 2024Danis AB, Gallagher AA, Anderson AN, Isakharov A, Beeson KA, Schnell E.serum: rabbit anti-c-fos, Cell Signaling 2250, RRID:AB_2247211, 1:300; rabbit anti-ΔFosB, Cell Signaling 14695, RRID:AB_2798577, 1:400; guinea pig anti-ZnT3, Synaptic Systems 197004, RRID:AB_2189667, 1:200; mouse

(13) Inhibition of STAT3 by S3I-201 suppress peritoneal fibroblast phenotype conversion and alleviate peritoneal fibrosisJournal of cellular and molecular medicineMay 1, 2024Song Q, Li H, Yan H, Yu Z, Li Z, Yuan J, Jiang N, Ni Z, Gu L, Fang W.Cell Signaling Technology (Danvers, USA). Antibodies to Cytokeratin (#10830‐1‐AP), Vimentin (#10366), Collagen I (#14695), α‐SMA (#14395) and CD45 (#20103‐1‐AP) were purchased from Proteintech (Rosemont, USA). Anti‐Factor VIII

(14) Genetic loss of the dopamine transporter significantly impacts behavioral and molecular responses to sub-chronic stress in miceFrontiers in Molecular NeuroscienceFebruary 29, 2024Avelina Petri, Abigail Sullivan, Kristen Allen, Benjamin D. Sachsone of the following primary antibodies overnight at 4°C: anti-Δ FosB (Cell Signaling Technologies, #14695, 1:500 dilution); anti-phospho-ERK (Cell Signaling Technologies, #4370, 1:300); anti-total-ERK (Cell Signaling Technologies

(15) ΔFosB is part of a homeostatic mechanism that protects the epileptic brain from further deteriorationFrontiers in Molecular NeuroscienceJanuary 12, 2024Jerome Clasadonte, Tania Deprez, Gabriel S. Stephens, Georges Mairet-Coello, Pierre-Yves Cortin, Maxime Boutier, Aurore Frey, Jeannie Chin, Marek Rajmanblocked in Odyssey blocking buffer (LI-COR), and ΔFosB was detected using ΔFosB antibody (Cell Signaling #14695S; dilution 1:2000). Actin B was used as loading control (LI-COR; dilution 1:10000). Secondary antibody

(16) Hippocampal ΔFosB expression is associated with cognitive impairment in a subgroup of patients with childhood epilepsiesFrontiers in NeurologyJanuary 11, 2024Chia-Hsuan Fu, Jason C. You, Carrie Mohila, Robert A. Rissman, Daniel Yoshor, Angela N. Viaene, Jeannie ChinRRID:AB_2336521). The antibody concentrations used were 1:200 for rabbit anti-ΔFosB antibody (Cell Signaling, Cat# 14695, RRID:AB_2798577) and 1:200 for goat anti-rabbit biotinylated antibody (Vector Laboratories, Cat# BA-1000,

(17) The chromatin remodeling protein BRG1 regulates HSC-myofibroblast differentiation and liver fibrosisCell Death DisDecember 14, 2023Yuwen Zhu, Aoqi Kang, Yameng Kuai, Yan Guo, Xiulian Miao, Li Zhu, Ming Kong, Nan LiWestern blot analyses were performed with anti-α-SMA (Abcam, ab7817), anti-collagen type I (Proteintech, 14695-1), anti-BRG1 (Cell Signaling Techy, 49306), anti-IGFBP5 (Abcam, ab254324), anti-Bat3 (Proteintech,

(18) A cytoskeleton-membrane interaction conserved in fast-spiking neurons controls movement, emotion, and memoryMolecular psychiatrySeptember 1, 2023Ma D, Sun C, Manne R, Guo T, Bosc C, Barry J, Magliery T, Andrieux A, Li H, Gu C.antibody (K14/16, NeuroMab). The rabbit monoclonal antibody: anti-Delta FosB (D3S8R) antibody (mAb #14695, Cell Signaling Technology, Inc). Rabbit polyclonal antibodies: the anti-MAP6 antibody (NBP214220, Novus Biologicals

(19) Neuroplastic changes in c-Fos, ΔFosB, BDNF, trkB, and Arc expression in the hippocampus of male Roman rats: differential effects of sexual activityHippocampusJuly 1, 2022Sanna F, Serra MP, Boi M, Bratzu J, Poddighe L, Sanna F, Carta A, Corda MG, Giorgi O, Melis MR, et al.0.1% Tween 20 (TBS‐T), for 60 min at room temperature. Rabbit monoclonal antibody against ΔFosB (Cat#14695; RRID:AB_2798577, Cell Signaling Technology), diluted 1:1000, rabbit polyclonal antisera against BDNF

(20) Repeated vagus nerve stimulation produces anxiolytic effects via upregulation of AMPAR function in centrolateral amygdala of male ratsNeurobiology of StressMay 1, 2022Shao-Qi Zhang, Zhi-Xuan Xia, Qiao Deng, Ping-Fen Yang, Li-Hong Long, Fang Wang, Jian-Guo Chenab208942, Cambridge, UK) and mouse anti-ΔFosB antibody (1:1000 dilution; Cell Signaling Technology, 14695, Denvars, MA, USA). Sections were then incubated with secondary antibodies of Alexa Fluor 488 Donkey anti-Mouse IgG (H + L) antibody

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eLife

eLife

Frontiers in behavioral neuroscience

Frontiers in behavioral neuroscience

Nature Metabolism

Delta FosB (D3S8R) Rabbit mAb from Cell Signaling Technology

Cell Signaling Technology

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Delta FosB (D3S8R) Rabbit mAb from Cell Signaling Technology

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