Fig 2: Representative images from western blot for the proteins (A) and relative expression levels of VEGFR2 (B), MMP1 (C), PPARγ (D), and IGF1 (E). **p < 0.01, vs the sham group. # p < 0.05, ## p < 0.01, vs the I/R group (n = 3).

Fig 3: 3D docking conformation of SAC with (A) KDR, (B) IGF1 (C) PPARG (D) MMP1, respectively. The green sticks represent SAC.

Fig 4: MMP1 is upregulated in HNSCC.A MMP1 expression profile across all tumor samples and paired normal tissues in TCGA. Compare with other primary tumor samples, MMP1 expression level was significantly upregulated in HNSCC samples. B Kaplan–Meier analysis of overall survival. Compared with patients with low MMP1 expression, those with high MMP1 expression had a significantly lower overall survival rate. C, D MMP1 expression in HNSCC samples and paired normal tissues (tumor/normal) from 53 patients were examined by qRT-PCR (brown for MMP1 down-regulation in tumor tissues compared to normal tissues while orange for up-regulation in tumor tissues) and those from another 92 patients were examined by immunohistochemistry staining. (−: negative; +: positive; ++: strongly positive).

Fig 5: Effects of C. sinensis extract pretreatment (25 and 50 μg/mL) for 4 h on the regulation of (A) mRNA expression of type 1 collagen (COL1A1) and elastin (ELN) and (B) mRNA expression of MMP9 and MMP1; protein levels of COL1A1 (C), ELN (D), MMP1 (E), and MPP9 (F) after exposure to UVB (25 and 50 mJ/cm2) in HFF1 cells (G). Representative immunoblotting image of each protein examined. The data are reported as the mean ± S.D. from two experiments performed in quadruplicates. Dashes to the right of the membrane bands show positions of prestained molecular mass markers. ** Significant vs. untreated control cells: p < 0.01; *** Significant vs. untreated control cells: p < 0.001; # Significant vs. untreated irradiated cells: p ≤ 0.05; ## Significant vs. untreated irradiated cells: p < 0.01.