Fig 1: E. chaffeensis colocalizes with Wnt receptor complex proteins early in infection. (A) E. chaffeensis colocalizes with Fzd2, 4, 5, 7, and 9 and LRP5 but not Fzd8. E. chaffeensis-infected (MOI 200) or mock-infected THP-1 cells were harvested 3 hpi, dual stained for E. chaffeensis (red) and respective Fzd or LRP antibodies (green), and visualized by confocal fluorescence microscopy. Nuclei are stained with DAPI (blue). Images are single 0.3-μm slices through the respective samples. Gridlines through merged image on far right represent stacked slices through Z plane visualized in the corresponding XZ and YZ orthogonal projections in order to demonstrate colocalization of red and green channels. Red box indicates inset of merged image. Scale bar = 10 μm. Images are representative of three independent experiments. (B) Table depicting positive or negative colocalization of E. chaffeensis and respective Fzd or LRP homologues within respective fields as determined by Mander’s overlap coefficient (MOC). Positive (“+”) colocalization was determined as an MOC of 0.6 or higher.