Fig 1: Consistent expression trends of IFI-16 and galectin-3(A) Pearson correlation heatmap illustrates the repeatability of samples within and between groups in myocytes overexpressing (OV) IFI-16 with the IFI16-pcdh-GFP+PURO plasmid compared to the control group using the pcdh-GFP+PURO plasmid (n = 3), all samples were subjected to 4-h hypoxia and nutrient deprivation (H4).(B) Volcano plots depict the differentially expressed genes between the OV-H4 and H4 groups. Red and green dots represent up-regulated (189 dots) and down-regulated (21 dots) transcripts, respectively (p < 0.05, [log2fc] > 1).(C) An analysis of disease association was performed on the different genes using disgenet.(D) Heatmap displays myocardial ischemia-related genes between the OV-H4 and Vector-H4 groups.(E and I) Western blot analysis of galectin-3 expression in AC16 myocytes during the in vitro hypoxia and nutrient deprivation for 0–6 h.(F and J) Western blot analysis of galectin-3 expression in IFI-16 knockdown AC16 cells in 4 h of hypoxia and nutrient deprivation in vitro model (H4).(G and K) Western blot analysis of galectin-3 expression in the hearts of the post-infarction myocardial remodeling mouse model.(H and L) Western blot analysis of galectin-3 expression in the hearts of IFI-16 knockdown mouse models.(M) A correlation analysis was performed to assess the relationship between IFI-16 and galectin-3 expression. Data are presented as mean ± SEM.