Fig 1: (Relative to Stage 3). MiR-3606-3p overexpression, ITGAV, GAB1, and TGFBR2 knockdown repress fibroblast migration. (A) Cell migration assays were evaluated by morphology and (B) relative closure distance of cell migration in primary fibroblasts with miR-3606-3p overexpression, ITGAV, GAB1, or TGFBR2 knockdown. Scale bar, 500 μm. (C) Venn diagram analysis of the overlap between DEGs and fibroblast migration genes. (D) Upregulated and downregulated fibroblast migration genes in miR-3606-3p-overexpressing fibroblasts compared with NC. (E) Elevated levels of ADA1 and NID2 in miR-3606-3p overexpression, ITGAV, GAB1, and TGFBR2 knockdown fibroblasts. (F–G) Cell migration assay results in primary fibroblasts with si-ADA1 and si-NID2. Scale bar, 500 μm. (H) Increased mRNA levels of fibrotic genes after si-ADA1 and si-NID2 treatment. The experiment was repeated three independently times in D-H. (I–J) Protein levels of ADA1, α-SMA, and collagen I were detected by western blotting. (K–L) Protein levels of NID2, α-SMA, and collagen I were detected by western blotting. The experiments were repeated in triplicate in I-L. (M−O) Diagram and live imaging to assess the cell migration rate in vivo. N = 6. Data in B, E, G, and H were evaluated by one-way ANOVA (Dunnett’s test). Data in D, J, and L were evaluated using the Student's t-test and corrected using the Benjamini-Hochberg procedure. Data in O were evaluated using the Student's t-test. Data represented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.