Fig 2: GRB7 partially rescues the effect of downregulation of TCF12 in HER2+ cells. A MTT assay, B colony formation assay and C EdU assay showed that GRB7 could partially reverse the inhibitory effect of TCF12 knockdown on cell proliferation. D Wound healing assays and E Transwell assays indicated that overexpression of GRB7 partially restored the inhibitory effect of TCF12 downregulation on cell migration and invasion. Data are shown as the mean ± SD, N ≥ 3. *p < 0.05, **p < 0.01, ***p < 0.001

Fig 3: TCF12 binds to the GRB7 promoter and promotes GRB7 expression. A Prediction of TFs that may regulate GRB7 expression by the Cistrome DB database. B The correlation between the expression of TCF12 and GRB7. C The potential binding sites of GRB7 were analyzed by JASPAR. D Agarose electrophoresis was used for chromatin immunoprecipitation (ChIP) analysis of TCF12 binding to the GRB7 promoter in SK-BR-3 cells. E Detailed fragments of TCF12 bound to the GRB7 promoter in SK-BR-3 cells were analyzed by ChIP. F Dual-luciferase reporter assays of the corresponding luciferase activities in SK-BR-3 cells. G Western blot and RT‒qPCR were used to analyze the mRNA and protein levels of TCF12 and GRB7 in SK-BR-3 and MDA-MB-453 cells after TCF12 knockdown. H Western blot and RT-qPCR were used to analyze the mRNA and protein levels of TCF12 and GRB7 in SK-BR-3 and MDA-MB-453 cells after TCF12 overexpression. Data are shown as the mean ± SD, N ≥ 3. *p < 0.05, **p < 0.01, ***p < 0.001. ns no significant difference